一种针对细胞外结构域保守序列的单克隆抗体可识别哺乳动物靶组织中的血管紧张素II AT1受体。

A monoclonal antibody to a conserved sequence in the extracellular domain recognizes the angiotensin II AT1 receptor in mammalian target tissues.

作者信息

Barker S, Marchant W, Ho M M, Puddefoot J R, Hinson J P, Clark A J, Vinson G P

机构信息

Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, London, U K.

出版信息

J Mol Endocrinol. 1993 Oct;11(2):241-5. doi: 10.1677/jme.0.0110241.

DOI:10.1677/jme.0.0110241

PMID:7507680

Abstract

We have generated hybridomas which secrete monoclonal antibodies to the AT1 subtype of the angiotensin II receptor (AT1 receptor). These were obtained after immunization of Balb C/c mice with synthetic peptides representing sequences from either the extracellular domain (residues 8-17) or the intracellular domain (residues 229-237) of the AT1 receptor. Hybridoma populations were first screened for the production of antibodies which bound to rat liver cells. Further selection, and cloning by limiting dilution, was carried out for antibodies which bound specifically to rat adrenal glomerulosa cells. Confirmation that the antibody designated 6313/G2 interacted with the angiotensin II receptor was obtained using COS-7 cells transfected with AT1A receptor cDNA. In particular, the initial characterization of 6313/G2 showed specific immunofluorescence of vascular endothelium.

摘要

我们已制备出能分泌抗血管紧张素 II 受体 1 型（AT1 受体）单克隆抗体的杂交瘤。这些杂交瘤是在用代表 AT1 受体细胞外结构域（第 8 - 17 位氨基酸残基）或细胞内结构域（第 229 - 237 位氨基酸残基）序列的合成肽免疫 Balb C/c 小鼠后获得的。首先筛选杂交瘤群体，以获得能与大鼠肝细胞结合的抗体。对于能特异性结合大鼠肾上腺球状带细胞的抗体，进一步进行了选择，并通过有限稀释法进行克隆。使用转染了 AT1A 受体 cDNA 的 COS - 7 细胞，证实了命名为 6313/G2 的抗体与血管紧张素 II 受体相互作用。特别是，6313/G2 的初步特性表明其对血管内皮有特异性免疫荧光。