Ogbunude P O, Dzimiri M M
Biological and Medical Research (MBC-03), King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia.
Int J Parasitol. 1993 Sep;23(6):803-7. doi: 10.1016/0020-7519(93)90078-d.
L. donovani promastigotes (MHOM/ET/67/HA3) transport adenosine by a route that is sensitive to inhibition by a nonspecific channel blocker, propranolol. At the logarithmic and stationary phases of growth, the transport of 1 microM-3H-adenosine was significantly inhibited (40-50%) by 100 microM-propranolol. In contrast, a strain of Leishmania donovani promastigotes clonally selected to grow in defined medium was only slightly (approximately 10%) inhibited at the logarithmic but not at the stationary phase. These results suggest that the differences in expression of adenosine in the parasites previously reported, may be related to uptake by a channel-like pathway in the promastigotes.
杜氏利什曼原虫前鞭毛体（MHOM/ET/67/HA3）通过一条对非特异性通道阻滞剂普萘洛尔的抑制敏感的途径转运腺苷。在对数生长期和稳定期，100微摩尔普萘洛尔可显著抑制（40%-50%）1微摩尔-3H-腺苷的转运。相比之下，一株经克隆选择在限定培养基中生长的杜氏利什曼原虫前鞭毛体在对数期仅受到轻微抑制（约10%），在稳定期则未受抑制。这些结果表明，先前报道的寄生虫中腺苷表达的差异可能与前鞭毛体中类似通道途径的摄取有关。
