短杆菌肽A的孔径尺寸。
The pore dimensions of gramicidin A.
作者信息
Smart O S, Goodfellow J M, Wallace B A
机构信息
Department of Crystallography, Birkbeck College, University of London, England.
出版信息
Biophys J. 1993 Dec;65(6):2455-60. doi: 10.1016/S0006-3495(93)81293-1.
Abstract
The ion channel forming peptide gramicidin A adopts a number of distinct conformations in different environments. We have developed a new method to analyze and display the pore dimensions of ion channels. The procedure is applied to two x-ray crystal structures of gramicidin that adopt distinct antiparallel double helical dimer conformations and a nuclear magnetic resonance (NMR) structure for the beta6.3 NH2-terminal to NH2-terminal dimer. The results are discussed with reference to ion conductance properties and dependence of pore dimensions on the environment.
摘要
形成离子通道的肽短杆菌肽A在不同环境中会呈现多种不同的构象。我们开发了一种新方法来分析和展示离子通道的孔径。该方法应用于短杆菌肽的两种X射线晶体结构，这两种结构呈现出不同的反平行双螺旋二聚体构象，以及β6.3 NH2末端到NH2末端二聚体的核磁共振（NMR）结构。结合离子传导特性以及孔径对环境的依赖性对结果进行了讨论。
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本文引用的文献
1
Ion-bond forms of the gramicidin a transmembrane channel.
Biophys J. 1984 Jan;45(1):114-6. doi: 10.1016/S0006-3495(84)84131-4.
2
Optimization by simulated annealing.
Science. 1983 May 13;220(4598):671-80. doi: 10.1126/science.220.4598.671.
3
X-ray analysis and protein structure.
Cold Spring Harb Symp Quant Biol. 1950;14:65-78. doi: 10.1101/sqb.1950.014.01.010.
4
GRAMICIDIN A. V. THE STRUCTURE OF VALINE- AND ISOLEUCINE-GRAMICIDIN A.
J Am Chem Soc. 1965 May 5;87:2011-20. doi: 10.1021/ja01087a027.
5
The permeation properties of small organic cations in gramicidin A channels.
Biophys J. 1993 Apr;64(4):1017-28. doi: 10.1016/S0006-3495(93)81467-X.
6
Solvent-accessible surfaces of proteins and nucleic acids.
Science. 1983 Aug 19;221(4612):709-13. doi: 10.1126/science.6879170.
7
Gramicidin channels.
Annu Rev Physiol. 1984;46:531-48. doi: 10.1146/annurev.ph.46.030184.002531.
8
An interaction between gramicidin and the sigma subunit of RNA polymerase.
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1045-8. doi: 10.1073/pnas.79.4.1045.
9
Conformation of the gramicidin A transmembrane channel: A 13C nuclear magnetic resonance study of 13C-enriched gramicidin in phosphatidylcholine vesicles.
J Mol Biol. 1980 Oct 15;143(1):1-19. doi: 10.1016/0022-2836(80)90121-7.
10
The gramicidin A transmembrane channel: a proposed pi(L,D) helix.
Proc Natl Acad Sci U S A. 1971 Mar;68(3):672-6. doi: 10.1073/pnas.68.3.672.
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