Bagnasco M, Franco M D, Lopez M, Nunes J, Lipcey C, Mawas C, Salamero J, Olive D
Center for Oncology and Experimental Therapy, INSERM U119, Marseille, France.
Hum Immunol. 1993 Nov;38(3):172-8. doi: 10.1016/0198-8859(93)90536-a.
We comparatively evaluated (Ca2+)i mobilization after triggering with a stimulatory pair of CD2 (CD2.9, anti-T11.1 + CD2.1, anti-T11.2) or CD3 mAbs in the differentiated T-cell line Jurkat, using INDO-1 labeling and cytofluorimetry. The results obtained showed different (Ca2+)i mobilization kinetics following CD2 or CD3 stimulation (the former being slower than the latter), not due to different association kinetics of mAbs. In a nonreciprocal manner, however, preliminary interaction with CD2.1 (anti-T11.2) followed by CD2.9 (anti-T11.1) induces a rapid (Ca2+)i rise, similar to CD3 stimulation, as shown by preincubation experiments. There is no interference between CD2.9 and CD2.1 mAb binding. CD2.1 mAb by itself is unable to induce (Ca2+)i mobilization; in addition, preincubation with CD2.1 mAb did not modify the CD2, CD3, CD45, or CD28 immunoprecipitation patterns. Triggering of the epitope recognized by CD2.1 mAb may favor, possibly via conformational changes of CD2 molecule or (Ca2+)i-unrelated metabolic effect(s), optimal signal transduction.
我们使用indo-1标记和细胞荧光测定法，在分化的t细胞系jurkat中，比较评估了用一对刺激性cd2单克隆抗体（cd2.9，抗t11.1 + cd2.1，抗t11.2）或cd3单克隆抗体触发后的（ca2 +）i动员情况。获得的结果显示，cd2或cd3刺激后（ca2 +）i动员动力学不同（前者比后者慢），这不是由于单克隆抗体的结合动力学不同所致。然而，以非互惠的方式，先用cd2.1（抗t11.2）进行初步相互作用，然后再用cd2.9（抗t11.1），会诱导（ca2 +）i迅速升高，类似于cd3刺激，预孵育实验表明了这一点。cd2.9和cd2.1单克隆抗体结合之间没有干扰。cd2.1单克隆抗体本身不能诱导（ca2 +）i动员；此外，用cd2.1单克隆抗体预孵育不会改变cd2、cd3、cd45或cd28的免疫沉淀模式。触发cd2.1单克隆抗体识别的表位可能有利于，可能通过cd2分子的构象变化或与（ca2 +）i无关的代谢效应，实现最佳信号转导。
