Shimane M, Tani K, Maruyama K, Takahashi S, Ozawa K, Asano S
Institute of Medical Science, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1994 Feb 28;199(1):26-32. doi: 10.1006/bbrc.1994.1187.
G-CSF (granulocyte colony-stimulating factor) is known to specifically stimulate the production and the functional activation of neutrophils. To investigate the intracellular signaling pathway of myeloid cells stimulated by G-CSF, we isolated new genes whose expression was induced by G-CSF. First of all, we constructed lambda gt10 cDNA library from G-CSF-stimulated mononuclear cells (MNC) of a chronic myelogenous leukemia (CML) patient (CML-MNC) and screened the cDNA library by a differential hybridization method. The 24 candidate clones which specifically hybridized with G-CSF-stimulated CML-MNC cDNA probes, but not with unstimulated CML-MNC cDNA probes, were obtained after 8 x 10(4) individual clones had been screened. One of these clones, GIG-1 (G-CSF-induced gene-1), was further characterized. The size of the GIG-1 mRNA was about 0.9kb. The GIG-1 mRNA was expressed mainly in the myeloid leukemic cell lines.
已知粒细胞集落刺激因子（G-CSF）能特异性刺激中性粒细胞的产生和功能激活。为了研究G-CSF刺激髓系细胞的细胞内信号通路，我们分离了受G-CSF诱导表达的新基因。首先，我们从一名慢性粒细胞白血病（CML）患者的G-CSF刺激的单核细胞（MNC）（CML-MNC）构建了λgt10 cDNA文库，并通过差异杂交方法筛选该cDNA文库。在筛选了8×10⁴个单个克隆后，获得了24个与G-CSF刺激的CML-MNC cDNA探针特异性杂交，但与未刺激的CML-MNC cDNA探针不杂交的候选克隆。其中一个克隆，GIG-1（G-CSF诱导基因-1），被进一步鉴定。GIG-1 mRNA的大小约为0.9kb。GIG-1 mRNA主要在髓系白血病细胞系中表达。
