Nozaki M, Mori M, Matsushiro A
Department of Microbial Genetics, Osaka University, Japan.
Gene. 1994 Jan 28;138(1-2):197-200. doi: 10.1016/0378-1119(94)90807-9.
To characterize the type-I keratin-encoding gene family around the mouse keratin 19-encoding gene (K19, EndoC), which encodes simple epithelial-type cytokeratin (CK), we screened a mouse genomic library by hybridization to a K19 cDNA probe. One clone of 16 kb contained the second to the sixth exons of K19 and the other keratin-encoding gene was located about 4 kb downstream from K19. Sequencing, Northern hybridization and genomic Southern blotting revealed that the downstream gene encodes the mouse K15 gene. This gene consists of eight exons and the positions of the introns coincide with those of other type-I keratin-encoding genes. The 5' upstream regions of the mouse and human K15 genes contain homologous sequences around the respective TATA boxes, suggesting that the same factors are involved in the regulation of their transcription.
为了鉴定小鼠角蛋白19编码基因（K19，EndoC）周围的I型角蛋白编码基因家族，该基因编码简单上皮型细胞角蛋白（CK），我们通过与K19 cDNA探针杂交筛选了小鼠基因组文库。一个16 kb的克隆包含K19的第二至第六外显子，另一个角蛋白编码基因位于K19下游约4 kb处。测序、Northern杂交和基因组Southern印迹分析表明，下游基因编码小鼠K15基因。该基因由八个外显子组成，内含子的位置与其他I型角蛋白编码基因的位置一致。小鼠和人类K15基因的5'上游区域在各自的TATA框周围含有同源序列，表明相同的因子参与了它们转录的调控。
