黏蛋白肽以及血型ABH和Lewis相关碳水化合物抗原在正常人结膜中的表达。

Expression of mucin peptide and blood group ABH- and Lewis-related carbohydrate antigens in normal human conjunctiva.

作者信息

Garcher C, Bara J, Bron A, Oriol R

机构信息

Department of Ophthalmology, University of Burgundy, Dijon, France.

出版信息

Invest Ophthalmol Vis Sci. 1994 Mar;35(3):1184-91.

PMID:7510273

Abstract

PURPOSE

The immunohistochemical characterization of mucin peptide antigens and identification of blood group ABH- and Lewis-related carbohydrate antigens expressed in epithelium of normal human conjunctiva.

METHODS

Immunoperoxidase characterization of conjunctival glycoproteins was performed using antibodies against peptide and saccharide moieties of gastrointestinal mucins on conjunctival biopsy specimens from 89 healthy individuals of known ABO and Lewis red cell phenotypes.

RESULTS

Ocular mucins had epitopes in common with the peptidic core of gastric mucin (M1 epitopes), but also contained sialylated saccharide chains like those of intestinal mucins. Anti-M1 gastric mucins, anti-type 1 precursor, and anti-T antibodies strongly stained the cytoplasm of goblet cells. Anti-Lea, anti-NeuAc-Lea, and anti-Leb antibodies stained both epithelial and goblet cells of Lewis-positive individuals only. Anti-H type 1, anti-H type 2, anti-A, and anti-B stained epithelial cells of ABH secretors. In addition, A-like epitopes independent of secretor phenotype were found in epithelial and goblet cells of all donors.

CONCLUSIONS

The conjunctival mucins cross-react with antibodies specific for digestive mucins and for blood group-related carbohydrate epitopes. The distributions of Lewis and secretor phenotypes in conjunctiva are: 19% Lewis-positive nonsecretors, 71% Lewis-positive secretors, and 10% Lewis-negative secretors. Lea and Leb antigens are present on epithelial cells of Lewis-positive nonsecretors and Lewis-positive secretors, respectively, as expected for antigens under control of Lewis and secretor genes. The secretor alpha-2-fucosyltransferase is not expressed on goblet cells, as described for the goblet cells of the distal rectal colonic mucosae. ABH antigens synthesized by the products of A1, A2, B, and Se genes and modulated by the presence of the product of the Lewis gene are found in epithelial cells. In addition, A-related epitopes independent of the A1-A2 subtype, secretor, and Lewis phenotypes also are present in both epithelial and goblet cells.

摘要

目的

对黏蛋白肽抗原进行免疫组织化学特征分析，并鉴定正常人结膜上皮中表达的ABH血型及Lewis相关碳水化合物抗原。

方法

使用针对胃肠道黏蛋白肽和糖部分的抗体，对89名已知ABO和Lewis红细胞表型的健康个体的结膜活检标本进行结膜糖蛋白的免疫过氧化物酶特征分析。

结果

眼黏蛋白具有与胃黏蛋白肽核心（M1表位）相同的表位，但也含有类似肠黏蛋白的唾液酸化糖链。抗M1胃黏蛋白、抗1型前体和抗T抗体强烈染色杯状细胞的细胞质。抗Lea、抗NeuAc-Lea和抗Leb抗体仅对Lewis阳性个体的上皮细胞和杯状细胞染色。抗H1型、抗H2型、抗A和抗B染色ABH分泌者的上皮细胞。此外，在所有供体的上皮细胞和杯状细胞中均发现了与分泌者表型无关的A样表位。

结论

结膜黏蛋白与针对消化性黏蛋白和血型相关碳水化合物表位的特异性抗体发生交叉反应。结膜中Lewis和分泌者表型的分布为：19%Lewis阳性非分泌者，71%Lewis阳性分泌者，10%Lewis阴性分泌者。正如Lewis和分泌者基因控制下的抗原所预期的那样，Lea和Leb抗原分别存在于Lewis阳性非分泌者和Lewis阳性分泌者的上皮细胞上。分泌者α-2-岩藻糖基转移酶在杯状细胞上不表达，如远端直肠结肠黏膜的杯状细胞所述。由A1、A2、B和Se基因产物合成并受Lewis基因产物存在调节的ABH抗原存在于上皮细胞中。此外，与A1-A2亚型、分泌者和Lewis表型无关的A相关表位也存在于上皮细胞和杯状细胞中。