Cordon-Cardo C, Reuter V E, Lloyd K O, Sheinfeld J, Fair W R, Old L J, Melamed M R
Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Cancer Res. 1988 Jul 15;48(14):4113-20.
Seven mouse monoclonal antibodies and the lectin from Ulex europaeus, detecting blood group specificities of the ABH and Lewis systems, have been used to define the expression and/or modulation of these antigenic structures in human normal urothelium and tumors of the urinary bladder. The reagents employed recognize the following blood group related antigens: A, B, H, Lewisa (Lea), Lewisb (Leb), Lewisx (Lex), Lewisy (Ley), and type 1 precursor chain. Immunohistochemical studies have demonstrated that these antigenic systems are differentially expressed in the urothelium of secretor and nonsecretor individuals. The normal urothelium of secretors is particularly rich in ABH blood group antigens as well as Leb and Ley specificities. Nonsecretors, however, either lack or show decreased and patchy expression of H, Leb, and Ley antigens. In general, areas affected by carcinoma in situ showed deletion of ABH, as did invasive carcinomas, as demonstrated by other investigators. This was not a universal observation, however, as variable expression of ABH antigens occurred in a few invasive tumors. Lex antigen was not expressed in normal urothelium except for occasional umbrella cells, but was demonstrated in the majority of invasive tumors, regardless of blood type and secretor status of the individuals studied. Ley determinant, which was poorly expressed in the normal urothelium of nonsecretor individuals, was found in all tumors analyzed. An accumulation of non-fucosylated precursor structure was also a feature of invasive carcinoma, particularly in secretor individuals. A panel of anti-blood group antibodies, encompassing A, B, Lex, Ley and type 1 precursor chain specificities for use in patients of known secretor status, may provide useful early markers of malignant change in the urothelium.
七种小鼠单克隆抗体以及来自欧洲荆豆的凝集素可检测ABH和Lewis系统的血型特异性，已被用于确定这些抗原结构在人正常膀胱上皮和膀胱肿瘤中的表达和/或调节情况。所使用的试剂可识别以下与血型相关的抗原：A、B、H、Lea、Leb、Lex、Ley以及1型前体链。免疫组织化学研究表明，这些抗原系统在分泌型和非分泌型个体的膀胱上皮中存在差异表达。分泌型个体的正常膀胱上皮中尤其富含ABH血型抗原以及Leb和Ley特异性抗原。然而，非分泌型个体要么缺乏H、Leb和Ley抗原，要么其表达减少且呈斑片状。一般来说，原位癌受累区域显示ABH缺失，浸润性癌也是如此，其他研究者已证实这一点。然而，这并非普遍现象，因为在一些浸润性肿瘤中ABH抗原存在可变表达。Lex抗原在正常膀胱上皮中除偶尔的伞细胞外不表达，但在大多数浸润性肿瘤中可检测到，无论所研究个体的血型和分泌状态如何。Ley决定簇在非分泌型个体的正常膀胱上皮中表达较弱，但在所有分析的肿瘤中均有发现。非岩藻糖基化前体结构的积累也是浸润性癌的一个特征，在分泌型个体中尤为明显。一组针对已知分泌状态患者的抗血型抗体，包括A、B、Lex、Ley和1型前体链特异性抗体，可能为膀胱上皮恶性变化提供有用的早期标志物。
