Tsurushima H, Yuasa S, Kawamura K, Nose T
Department of Neurological Surgery, University of Tsukuba, Ibaraki, Japan.
Neurosci Res. 1993 Nov;18(2):109-20. doi: 10.1016/0168-0102(93)90014-h.
It is believed that 'cell-adhesion molecules' and neurotrophic factors play important roles in the host-graft interactions during the reconstruction of injured brain by neural transplantation. In this study, we have examined the expression of such molecules and factors during the migration and differentiation of grafted Purkinje and granule cells in the adult rat cerebellum. Cerebellar primordium at the 14th gestational day (E14) was transplanted into adult rat cerebellum. Purkinje cells which had migrated from the grafted tissue into the host molecular layer were identified immunohistochemically with a specific marker, anti-spot 35 antibody, as well as by labeling them with bromodeoxyuridine (BrdU) during their final mitotic period. In the grafted site, transient expression of a neuron-glia cell adhesion molecule, tenascin, was detected immunohistochemically. This molecule was expressed transiently in the host tissue adjacent to the migratory Purkinje cells, as well as within the grafted tissue. Tenascin was not detected in intact host tissue apart from the grafted tissue. In the light of tenascin expression in the migratory process of Purkinje and granule cells during cerebellar development, the induction of this molecule in the host tissue might be involved in the migration of grafted Purkinje and granule cells. Furthermore, gene expression of brain-derived neurotrophic factor (BDNF) was found by in situ hybridization and the expression of NGF receptor was found immunohistochemically in the areas where grafted Purkinje and granule cells developed. These findings suggest the involvement of the neurotrophic factor in the growth and differentiation of the grafted cerebellar primordium.
人们认为，“细胞黏附分子”和神经营养因子在神经移植重建受损大脑的宿主-移植物相互作用中发挥着重要作用。在本研究中，我们检测了成年大鼠小脑内移植的浦肯野细胞和颗粒细胞迁移与分化过程中这些分子和因子的表达。将妊娠第14天（E14）的小脑原基移植到成年大鼠小脑中。从移植组织迁移到宿主分子层的浦肯野细胞通过一种特异性标记物抗斑点35抗体进行免疫组织化学鉴定，以及在其最后有丝分裂期用溴脱氧尿苷（BrdU）进行标记。在移植部位，通过免疫组织化学检测到一种神经元-胶质细胞黏附分子腱生蛋白的瞬时表达。该分子在与迁移的浦肯野细胞相邻的宿主组织以及移植组织内瞬时表达。除移植组织外，在完整的宿主组织中未检测到腱生蛋白。鉴于腱生蛋白在小脑发育过程中浦肯野细胞和颗粒细胞迁移过程中的表达情况，宿主组织中该分子的诱导可能参与了移植的浦肯野细胞和颗粒细胞的迁移。此外，通过原位杂交发现了脑源性神经营养因子（BDNF）的基因表达，并且在移植的浦肯野细胞和颗粒细胞发育的区域通过免疫组织化学发现了NGF受体的表达。这些发现表明神经营养因子参与了移植的小脑原基的生长和分化。
