Pujol F H, Liprandi F, Alarcón de Noya B, Matos C, Cesari I M
Laboratorio de Immunoparasitología, Universidad Central de Venezuela, Caracas.
Trop Med Parasitol. 1993 Dec;44(4):289-94.
Eight IgM monoclonal antibodies (mAbs) obtained from a Schistosoma mansoni chronically infected mouse immunized twice with adult worms butanolic extract (BE) and soluble egg antigen (SEA) were characterized by immunochemical methods. An intense cross-reactivity between different developmental stages was observed by indirect immunofluorescence assay (IIFA) with five anti-SEA mAbs. These mAbs appeared to recognize glycosidic residues, as suggested by 1) the inhibition of their reactivity by periodate oxidation of SEA, 2) the multiple polypeptide recognition in radioimmunoprecipitation and immunoblot assays and 3) reactivity with KLH. Anti-SEA mAbs were able to mediate in vitro killing of schistosomula but they were not consistently able to mediate passive transfer immunity in vivo. Three of anti-SEA mAbs were suitable for the performance of a sandwich ELISA for antibody detection in S. mansoni infected patients, allowing an increase in the signal to noise ratio as compared to the direct ELISA SEA method. Three anti-BE mAbs showed a more stage restricted pattern of antigen recognition by IIFA. Only one out of three seemed to be directed against glycan residues, but the other mAbs showed a plural pattern of polypeptide recognition on BE immunoblot, suggesting that repeated epitopic motifs are also present in different proteins within the same parasite developmental stage.
从一只用曼氏血吸虫成虫丁醇提取物（BE）和可溶性虫卵抗原（SEA）免疫两次的慢性感染小鼠中获得了8种IgM单克隆抗体（mAb），并用免疫化学方法对其进行了表征。通过间接免疫荧光分析（IIFA）观察到，5种抗SEA mAb在不同发育阶段之间存在强烈的交叉反应。这些mAb似乎识别糖苷残基，这是由以下几点表明的：1）SEA经高碘酸盐氧化后其反应性受到抑制；2）在放射免疫沉淀和免疫印迹分析中对多种多肽的识别；3）与钥孔血蓝蛋白（KLH）的反应性。抗SEA mAb能够在体外介导对童虫的杀伤，但它们在体内并不能始终介导被动转移免疫。其中3种抗SEA mAb适用于夹心ELISA法检测曼氏血吸虫感染患者的抗体，与直接ELISA SEA法相比，可提高信噪比。3种抗BE mAb通过IIFA显示出更具阶段限制性的抗原识别模式。3种mAb中只有1种似乎针对聚糖残基，但其他mAb在BE免疫印迹上显示出多种多肽识别模式，这表明在同一寄生虫发育阶段的不同蛋白质中也存在重复的表位基序。
