Abe M, Tasaki K, Matsuda S, Sakuma H, Wakasa H
Department of Pathology, Fukushima Medical College, Japan.
Arch Pathol Lab Med. 1994 Mar;118(3):303-7.
To investigate the clonal origin of a case of CD5+B-intermediate lymphocytic lymphoma terminating in plasmacytoid differentiation, we analyzed the immunophenotype and immunoglobulin gene rearrangements in the first and second lymph nodes from which biopsy specimens were taken. Immunohistochemical study revealed that both neoplasms have the same immunoglobulin light chain type (kappa-light chain). Immunoglobulin gene analysis using the Southern blot method revealed the identical immunoglobulin heavy chain and kappa-light chain gene rearrangements in both neoplasms. These findings suggest that both neoplasms are derived from a single clonal B cell. The present case may help a further understanding of CD5+B-lymphocyte differentiation pathway.
为研究一例最终发生浆细胞样分化的CD5⁺B中间淋巴细胞淋巴瘤的克隆起源，我们分析了首次及第二次取活检标本的淋巴结的免疫表型和免疫球蛋白基因重排。免疫组织化学研究显示，两种肿瘤具有相同的免疫球蛋白轻链类型（κ轻链）。采用Southern印迹法进行的免疫球蛋白基因分析显示，两种肿瘤中免疫球蛋白重链和κ轻链基因重排相同。这些发现提示，两种肿瘤均来源于单个克隆性B细胞。本病例可能有助于进一步了解CD5⁺B淋巴细胞分化途径。
