一种在冰冻切片中显示嗜酸性粒细胞的简单方法。
A simple method for demonstration of eosinophils in frozen sections.
作者信息
Furgał A, Litwin J A
机构信息
Department of Histology, Jagiellonian University School of Medicine, Kraków, Poland.
出版信息
Folia Histochem Cytobiol. 1993;31(4):197-9.
Tissue eosinophils can be easily and specifically demonstrated in frozen sections after substrateless incubation with 3,3'-diaminobenzidine (DAB) at neutral pH. Under such conditions, other cells possessing endogenous peroxidatic activity (neutrophils, macrophages) do not stain, whereas staining of mitochondria-rich cells due to cytochrome oxidase activity can be avoided by a short prefixation of sections in glutaraldehyde-containing fixatives. The method can be useful in cases when a rapid screening of tissue eosinophils is required.
在中性pH条件下，将组织与3,3'-二氨基联苯胺（DAB）进行无底物孵育后，可在冷冻切片中轻松且特异性地显示组织嗜酸性粒细胞。在这种条件下，具有内源性过氧化物酶活性的其他细胞（中性粒细胞、巨噬细胞）不会染色，而通过在含戊二醛的固定剂中对切片进行短暂预固定，可以避免因细胞色素氧化酶活性导致的富含线粒体的细胞染色。当需要快速筛查组织嗜酸性粒细胞时，该方法可能会很有用。
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