Ang H L, Ivell R, Walther N, Nicholson H, Ungefroren H, Millar M, Carter D, Murphy D
Department of Molecular Biology, University of Hamburg, Germany.
J Endocrinol. 1994 Jan;140(1):53-62. doi: 10.1677/joe.0.1400053.
The bovine oxytocin gene has been expressed in the testes of two independent transgenic mouse lines. Hybridization and RNase protection analysis showed that the oxytocin transgene was transcribed from the normal functional promoter in the Sertoli cells of the seminiferous tubules in a developmentally regulated manner. Immunohistochemistry indicated that both oxytocin and neurophysin epitopes were expressed together in the Sertoli cells at stages I-V and X-XII of the cycle of the seminiferous epithelium. Furthermore, analysis with high-performance liquid chromatography showed that there was a tenfold increase in the amount of amidated oxytocin present in testicular extracts from the transgenic mice. However, there appeared to be no detectable effect of this overproduction of hormone on testicular morphology or fertility parameters. A significant decrease by 50% was detected only in the levels of intratesticular testosterone and dihydrotestosterone. The results point to a local paracrine role for oxytocin in the modulation of Leydig cell function.
牛催产素基因已在两个独立的转基因小鼠品系的睾丸中表达。杂交和核糖核酸酶保护分析表明，催产素转基因在生精小管的支持细胞中以发育调控的方式从正常功能启动子转录。免疫组织化学表明，在生精上皮周期的I-V期和X-XII期，催产素和神经垂体素表位在支持细胞中共同表达。此外，高效液相色谱分析表明，转基因小鼠睾丸提取物中酰胺化催产素的含量增加了十倍。然而，这种激素的过量产生似乎对睾丸形态或生育参数没有可检测到的影响。仅在睾丸内睾酮和双氢睾酮水平上检测到显著降低50%。结果表明催产素在调节睾丸间质细胞功能中具有局部旁分泌作用。
