Löning T, Schlechte H, Friedrichs K, Schnoor D, Ditscherlein G, Bommer G, Löning S
Abteilung für Gynäk, Histopathologie und Elektronenmikroskopie, Universitäts-Frauenklinik, Hamburg.
Verh Dtsch Ges Pathol. 1993;77:117-8.
DNA and paraffin material of more than 100 tumors of prostate, bladder and female genital organs were analyzed for p53 aberrations and compared with normal tissues by immunohistochemistry, PCR of p53 exons 5-8 and TGGE. While normal tissues, precancerous and borderline lesions, and well differentiated carcinomas usually showed wild type p53 and negative immunostaining, high grade and/or high stage carcinomas often revealed mutant p53 (rate of mutation in exon 8 >> 7 >> 6 >> 5) and/or p53 accumulation. Accumulation of p53 protein in the absence of detectable mutant p53 was recognized more often in prostate cancer than in any other tumor examined. Although p53 aberration probably represents a late molecular event in cancerogenesis, its detection may be of clinical interest as genetic footprint in recurrent and metastatic disease.
采用免疫组织化学、p53基因第5-8外显子的聚合酶链反应（PCR）以及变性梯度凝胶电泳（TGGE）技术，对100多例前列腺、膀胱及女性生殖器官肿瘤的DNA和石蜡材料进行p53基因异常分析，并与正常组织作比较。正常组织、癌前病变及临界病变以及高分化癌通常显示野生型p53且免疫染色阴性，而高级别和/或高分期癌常显示p53突变（第8外显子的突变率>>第7外显子>>第6外显子>>第5外显子）和/或p53蛋白积聚。在未检测到p53突变的情况下，p53蛋白积聚在前列腺癌中比在所检测的任何其他肿瘤中更常见。尽管p53基因异常可能是肿瘤发生过程中的一个晚期分子事件，但其检测作为复发和转移性疾病的基因印记可能具有临床意义。
