Kopp H, Masters J R, Wieland W, Hofstaedter F, Buettner R
Institut für Pathologie der Universität Regensburg.
Verh Dtsch Ges Pathol. 1993;77:129-32.
The sensitivity of prostate epithelial cells to androgens is mediated by the androgen receptor (AR). Analyses of cell lines in vitro and prostate carcinomas in vivo provide evidence that loss of AR expression parallels the loss of sensitivity to androgens. The aim of our work is to analyze the genetic control elements of the AR gene and answer the question of why these elements fail to function in hormone-insensitive prostate cancer cells. Therefore, we have cloned and fully sequenced a 5700 base pair sequence 5'-upstream of the AR gene. This DNA fragment contains promoter and enhancer elements that are active in androgen-sensitive but inactive in androgen-insensitive prostate cancer cell lines.
前列腺上皮细胞对雄激素的敏感性是由雄激素受体（AR）介导的。体外细胞系分析和体内前列腺癌分析均提供证据表明，AR表达的丧失与对雄激素敏感性的丧失平行。我们研究的目的是分析AR基因的遗传控制元件，并回答这些元件为何在激素不敏感的前列腺癌细胞中无法发挥作用的问题。因此，我们克隆了AR基因5'上游一个5700碱基对的序列并进行了全序列测定。该DNA片段包含在雄激素敏感的前列腺癌细胞系中具有活性但在雄激素不敏感的前列腺癌细胞系中无活性的启动子和增强子元件。
