Robertson M N, Buseyne F, Schwartz O, Rivière Y
Unité de Virologie et Immunologie Cellulaire, Institut Pasteur, Paris.
AIDS Res Hum Retroviruses. 1993 Dec;9(12):1217-23. doi: 10.1089/aid.1993.9.1217.
In the classic model of antigen processing and presentation, viral antigens must be synthesized within the cytoplasm of infected cells to be processed and presented to CD8+, MHC class I-restricted cytotoxic T lymphocytes (CTLs). We have examined the utility of a retroviral vector (pNeoNef) expressing the human immunodeficiency virus type (HIV-1)Lai Nef protein for the development of target cells to study HIV-specific CTLs. Autologous Epstein-Barr-transformed B cell lines (EBV-B cells) transduced with pNeoNef were efficiently lysed by CTL lines from donors capable of lysing EBV-B cells infected with a recombinant vaccinia virus (rVV) expressing Nef. Also, the transduced cells were efficient stimulator cells for the generation of Nef-specific CTL lines. The CTL lines thus generated recognized the same epitopes as CTL lines from the same donor generated by nonspecific stimulation. The use of similar cell lines transduced with retroviral vectors expressing HIV proteins may be useful in the study of CTLs in HIV-infected donors and in the study of the ability of candidate vaccines, including rVV, to induce HIV-specific CTLs. As antigen-presenting cells, the cell lines may be useful in the generation of antigen-specific CTL lines.
在经典的抗原加工与呈递模型中，病毒抗原必须在被感染细胞的细胞质内合成，以便进行加工并呈递给CD8⁺、受MHC I类分子限制的细胞毒性T淋巴细胞（CTL）。我们研究了一种表达人类免疫缺陷病毒1型（HIV-1）赖氏株Nef蛋白的逆转录病毒载体（pNeoNef）在开发用于研究HIV特异性CTL的靶细胞方面的效用。用pNeoNef转导的自体爱泼斯坦-巴尔病毒转化的B细胞系（EBV-B细胞）被来自能够裂解感染了表达Nef的重组痘苗病毒（rVV）的EBV-B细胞的供体的CTL系有效裂解。此外，转导的细胞是产生Nef特异性CTL系的有效刺激细胞。这样产生的CTL系识别的表位与由非特异性刺激产生的来自同一供体的CTL系识别的表位相同。使用用表达HIV蛋白的逆转录病毒载体转导的类似细胞系可能有助于研究HIV感染供体中的CTL，以及研究包括rVV在内的候选疫苗诱导HIV特异性CTL的能力。作为抗原呈递细胞，这些细胞系可能有助于产生抗原特异性CTL系。
