Koch T, Duncker H P, Klein A, Schlotzer E, Peskar B M, van Ackern K, Neuhof H
Institut für Anaesthesiologie und Operative Intensivmedizin, Klinikum der Stadt Mannheim, Fakultät für Klinische Medizin, Universität Heidelberg.
Infusionsther Transfusionsmed. 1993 Dec;20(6):291-300. doi: 10.1159/000222864.
The aim of this study was to investigate whether the pulmonary response to inflammatory stimulation, resulting in increased vascular resistance and permeability, could be attenuated by short-term infusion of triglycerides containing omega-3 fatty acids. With the concept of altering the composition of membrane phospholipids in such a manner that stimulation resulted in the release of less vasoconstrictive and permeability-enhancing metabolites of eicosapentaenoic acid instead of those of arachidonic acid (AA), the parenteral application of a lipid emulsion prepared from fish oil (Omegavenös) was tested in comparison with a soy oil preparation (Lipovenös).
Isolated lungs from anesthetized rabbits were ventilated and recirculatingly perfused (200 ml/min) with 200 ml cell-free buffer solution to which either 2 ml saline (controls, n = 6), 2 ml Lipovenös 10% (n = 6) or 2 ml Omegavenös 10% (n = 6) were added. To study the possible metabolic alterations in states of an enhanced AA turnover, lungs of each group were stimulated with smaller doses of A23187 (10(-8) M) during the 180-min lipid perfusion period, followed by a 10 times higher calcium ionophore A23187 (10(-7) M) challenge after washing out the lipids by exchange of perfusion fluid. Pulmonary artery pressure (PAP) and the lung weight gain indicating edema formation were monitored, and eicosanoids were analyzed in samples of the perfusate.
Upon A23187 injection lung weight gain and PAP increase were significantly reduced (50%) in Omegavenös-perfused lungs in comparison with controls and Lipovenös treatment. The vascular reactions were accompanied by a shifting from LTC4 to LTC5 during and after Omegavenös perfusion.
The data demonstrate that omega-3 fatty acids seem to be incorporated into the phospholipid pool of the pulmonary tissue, even after short-term infusion (3 h) resulting in an attenuated pressure reaction and edema formation due to an altered spectrum of metabolites in the case of inflammatory stimulation.
本研究旨在探讨短期输注含ω-3脂肪酸的甘油三酯是否能减轻肺部对炎症刺激的反应，这种反应会导致血管阻力和通透性增加。基于这样一种概念，即通过改变膜磷脂的组成，使刺激导致释放出较少的二十碳五烯酸血管收缩和通透性增强代谢产物，而非花生四烯酸（AA）的此类代谢产物，将由鱼油制备的脂质乳剂（Omegavenös）与大豆油制剂（Lipovenös）进行对比，测试其肠外应用效果。
对麻醉兔的离体肺进行通气，并以200ml/min的速度用200ml无细胞缓冲溶液进行再循环灌注，向该溶液中分别加入2ml生理盐水（对照组，n = 6）、2ml 10%的Lipovenös（n = 6）或2ml 10%的Omegavenös（n = 6）。为研究AA周转增强状态下可能的代谢变化，在180分钟的脂质灌注期内，用较小剂量的A23187（10⁻⁸M）刺激每组的肺，然后在通过更换灌注液洗去脂质后，用高10倍的钙离子载体A23187（10⁻⁷M）进行激发。监测肺动脉压（PAP）和表明水肿形成的肺重量增加情况，并对灌注液样本中的类花生酸进行分析。
与对照组和Lipovenös治疗组相比，用Omegavenös灌注的肺在注射A23187后，肺重量增加和PAP升高显著降低（50%）。在Omegavenös灌注期间及之后，血管反应伴随着从LTC4向LTC5的转变。
数据表明，即使在短期输注（3小时）后，ω-3脂肪酸似乎也能融入肺组织的磷脂池，在炎症刺激时，由于代谢产物谱的改变，导致压力反应和水肿形成减弱。
