Kabelitz D, Ackermann T, Hinz T, Davodeau F, Band H, Bonneville M, Janssen O, Arden B, Schondelmaier S
Paul Ehrlich Institute, Langen, Germany.
J Immunol. 1994 Mar 15;152(6):3128-36.
The gamma delta TCR is expressed on 1 to 5% of CD3+ human peripheral blood T lymphocytes. The majority of peripheral blood gamma delta T cells expresses V gamma 9 paired with V delta 2; this subset strongly responds to certain microbial ligands. Other gamma delta T cell subsets with unknown Ag specificity expressing different V gamma elements are present in peripheral blood and lymphoid tissue. We describe a new anti-human V gamma mAb termed 23D12 with unusual specificity. As revealed by analysis of a large number of T cell clones and transfectants expressing molecularly well-defined gamma delta TCR, mAb 23D12 recognized several, but not all, members of the human V gamma 1 family, specifically V gamma 2, V gamma 3, and V gamma 4 but not V gamma 5 or V gamma 8. In combination with available mAb against V gamma 4, mAb 23D12 was used to identify V gamma 2- or V gamma 3-bearing cells. On average, 23D12+ cells accounted for 18% of peripheral blood gamma delta T cells and 56% of postnatal gamma delta thymocytes. In combination with anti-V gamma 9 mAb, mAb 23D12 23D12 identified gamma delta cells expressing V elements other than V gamma 2, V gamma 3, V gamma 4, or V gamma 9. Such cells are detectable in peripheral blood and postnatal thymus. Using mAb 23D12, we also confirmed the appearance of two distinct TCR gamma-chains on the surface of some gamma delta T cells.
γδT细胞受体在1%至5%的CD3⁺人外周血T淋巴细胞上表达。大多数外周血γδT细胞表达与Vδ2配对的Vγ9；该亚群对某些微生物配体有强烈反应。外周血和淋巴组织中存在表达不同Vγ元件、抗原特异性未知的其他γδT细胞亚群。我们描述了一种具有独特特异性的新型抗人Vγ单克隆抗体，称为23D12。通过对大量表达分子定义明确的γδT细胞受体的T细胞克隆和转染子进行分析发现，单克隆抗体23D12识别人类Vγ1家族的几个成员，但不是全部，具体为Vγ2、Vγ3和Vγ4，但不识别Vγ5或Vγ8。与现有的抗Vγ4单克隆抗体联合使用时，单克隆抗体23D12可用于鉴定携带Vγ2或Vγ3的细胞。平均而言，23D12⁺细胞占外周血γδT细胞的18%，占出生后γδ胸腺细胞的56%。与抗Vγ9单克隆抗体联合使用时，单克隆抗体23D12可识别表达除Vγ2、Vγ3、Vγ4或Vγ9之外的V元件的γδ细胞。此类细胞在外周血和出生后胸腺中均可检测到。使用单克隆抗体23D12，我们还证实了一些γδT细胞表面出现了两条不同的TCRγ链。
