Donckier J, Hanet C, Stoleru L, Van Mechelen H, Galanti L, Hayashida W, Keyeux A, Ketelslegers J M, Pouleur H
Division of Endocrinology, University Hospital of Mont-Godinne, Yvoir, Belgium.
J Cardiovasc Pharmacol. 1994 Feb;23(2):212-9.
We assess hemodynamic, vascular, and hormonal effects of endothelin-1 (ET-1) at pathophysiologic levels on normal and ischemic myocardium. Thirty conscious chronically instrumented dogs were studied before, during, and after a 10-min coronary artery occlusion (CAO) performed either during ET-1 infusion (2.5 ng/kg.min, n = 15) or during placebo infusion (n = 15). ET-1 infusion produced an increase in plasma ET-1 (from 1.3 +/- 0.1 to 11.5 +/- 1.1 pM, p < 0.0001) during CAO (pathophysiologic value). Left anterior descending artery (LAD) blood flow (measured by Doppler flow probe) decreased similarly during CAO with ET-1 or placebo (p = 0.0001, NS, ET-1 vs. placebo). Both endocardial and epicardial blood flows in ischemic regions also decreased (p = 0.0001) during CAO but were threefold greater with ET-1 than with placebo (endocardium 42 +/- 7 vs. 14 +/- 2 ml/min/100 g, p = 0.003). No significant difference in myocardial blood flows between groups was observed in control regions. CAO produced increases (p < 0.005) in heart rate (HR), mean aortic pressure (AOP), and ventricular pressures but no change in atrial pressures. The changes in these parameters were comparable in the ET-1 and placebo groups. Despite the greater residual flow during CAO, however, ET-1 decreased the function of the ischemic zone during reperfusion as assessed by systolic shortening (p < 0.05). Atrial natriuretic factor (ANF), unchanged during CAO with placebo, increased from 38.3 +/- 6.1 to 53.3 +/- 10 pM with ET-1 (p = 0.02).(ABSTRACT TRUNCATED AT 250 WORDS)
我们在病理生理水平上评估内皮素-1（ET-1）对正常和缺血心肌的血流动力学、血管及激素影响。对30只长期植入仪器的清醒犬进行研究，在静脉输注ET-1（2.5 ng/kg·min，n = 15）或安慰剂（n = 15）期间，于冠状动脉闭塞（CAO）前、CAO期间（持续10分钟）及CAO后进行观察。在CAO（病理生理值）期间，ET-1输注使血浆ET-1升高（从1.3±0.1升至11.5±1.1 pM，p < 0.0001）。左前降支动脉（LAD）血流（通过多普勒血流探头测量）在CAO期间，ET-1组与安慰剂组下降程度相似（p = 0.0001，无显著差异，ET-1组与安慰剂组比较）。CAO期间，缺血区域的心内膜和心外膜血流均下降（p = 0.0001），但ET-1组下降幅度比安慰剂组大三倍（心内膜：42±7 vs. 14±2 ml/min/100 g，p = 0.003）。对照组区域心肌血流在两组间无显著差异。CAO使心率（HR）、平均主动脉压（AOP）和心室压力升高（p < 0.005），但心房压力无变化。这些参数的变化在ET-1组和安慰剂组中相当。然而，尽管CAO期间ET-1组残余血流较多，但再灌注时ET-1组缺血区功能下降，收缩期缩短评估结果显示（p < 0.05）。心房利钠因子（ANF）在安慰剂组CAO期间无变化，ET-1组则从38.3±6.1升至53.3±10 pM（p = 0.02）。（摘要截断于250字）
