Bernd A, Jackel C, Dold K, Görmar F, Theilig C, Breuer M, Ramirez-Bosca A, Bereiter-Hahn J, Holzmann H
Abteilung I, Johann Wolfgang Goethe-Universität, Frankfurt/Main, Fed. Rep. of Germany.
Arzneimittelforschung. 1994 Feb;44(2):137-40.
The mode of topical action of minoxidil (U-10,858, CAS 38304-91-5) ist not entirely clear. The influence of minoxidil on the ultimate differentiation of keratinocytes was investigated. Human keratinocytes (HaCaT-cells) were incubated with minoxidil containing culture medium (10-250 micrograms/ml). Minoxidil inhibited in a concentration dependent manner cell mobility whereas the adhesion area and the cell circumference were increased. The minoxidil treated cultures had a higher desmosome density compared to parallel control cultures and they expressed the suprabasal keratins 1 and 10 (indicating progress in differentiation) earlier and to a larger extent than the controls. Keratins were revealed immunohistochemically and by two-dimensional polyacrylamide gel electrophoresis. The results suggest that minoxidil supports the differentiation of human keratinocytes.
米诺地尔（U - 10,858，化学物质登记号38304 - 91 - 5）的局部作用方式尚不完全清楚。研究了米诺地尔对角质形成细胞最终分化的影响。将人角质形成细胞（HaCaT细胞）与含有米诺地尔的培养基（10 - 250微克/毫升）一起孵育。米诺地尔以浓度依赖性方式抑制细胞迁移，而黏附面积和细胞周长增加。与平行对照培养物相比，经米诺地尔处理的培养物具有更高的桥粒密度，并且它们比对照更早且更大程度地表达基底上层角蛋白1和角蛋白10（表明分化进展）。通过免疫组织化学和二维聚丙烯酰胺凝胶电泳揭示角蛋白。结果表明米诺地尔支持人角质形成细胞的分化。
