Garbarsch C, Matthiessen M E, Olsen B E, Moe D, Kirkeby S
Institute of Medical Anatomy A, Panum Institute, University of Copenhagen, Denmark.
Histochem J. 1994 Feb;26(2):110-8. doi: 10.1007/BF00157959.
The immunohistochemical localization of heparan sulphate, collagen type I, III and IV, laminin, tenascin, plasma- and cellular fibronectin was studied in tooth germs from human fetuses. The lamina basalis ameloblastica or membrana preformativa, which separates the pre-ameloblasts from the pre-dentin and dentin, contained heparan sulphate, collagen type IV, laminin and fibronectin. Enamel reacted with antifibronectin, but the reaction varied depending on the type of fibronectin and the source of antibody. In early pre-dentin, collagen type I, laminin, tenascin and fibronectin were present. In late pre-dentin and dentin collagen type I was found in intertubular dentin and in the zone between enamel and dentin. The close relationship between collagen type I in dentin and fibronectin in immature enamel is interesting, as it may contribute to the stabilization of the amelodentinal interface. In dental pulp, collagen type IV and laminin were found in the endothelial basement membranes. Collagen type I and III, tenascin and fibronectin were localized to the mesenchymal intercellular matrix. The results of this study have supported the assumption that the lamina basalis ameloblastica is a basement membrane, and have lead to the suggestion that ameloblasts are producers of fibronectin or a fibronectin-like substance.
研究了硫酸乙酰肝素、I型、III型和IV型胶原、层粘连蛋白、腱生蛋白、血浆纤维连接蛋白和细胞纤维连接蛋白在人类胎儿牙胚中的免疫组织化学定位。将成釉细胞与前期牙本质和牙本质分隔开的成釉细胞基底膜或前期形成膜含有硫酸乙酰肝素、IV型胶原、层粘连蛋白和纤维连接蛋白。釉质与抗纤维连接蛋白发生反应，但反应因纤维连接蛋白的类型和抗体来源而异。在早期前期牙本质中，存在I型胶原、层粘连蛋白、腱生蛋白和纤维连接蛋白。在晚期前期牙本质和牙本质中，I型胶原存在于管间牙本质以及釉质和牙本质之间的区域。牙本质中的I型胶原与未成熟釉质中的纤维连接蛋白之间的密切关系很有趣，因为它可能有助于釉牙本质界的稳定。在牙髓中，IV型胶原和层粘连蛋白存在于内皮基底膜中。I型和III型胶原、腱生蛋白和纤维连接蛋白定位于间充质细胞间基质。本研究结果支持了成釉细胞基底膜是一种基底膜的假设，并提示成釉细胞是纤维连接蛋白或类纤维连接蛋白物质的产生者。
