Suzuki S, Kita-Tsukamoto K, Fukagawa T
Faculty of Agriculture, Kochi University, Japan.
Microbios. 1994;77(311):101-9.
The 16S rRNA of the tributyltin resistant marine bacterium, strain M-1, was partly sequenced to confirm the taxonomic status. The results indicated that this bacterium should be classified under the genus Alteromonas, instead of a previous report in which this strain was identified as a Vibrio. The genome size of this strain was also measured by pulsed field gel electrophoresis (PFGE) using a contoureclamped homogeneous electric field. The strain was found to contain a genome size of 2,240 kilo base pairs, whereas Alteromonas nigrifaciens and Shewanella putrefaciens had 2,040 and 2,383 kilo base pairs, respectively. This is the first report of the genome sizing of the genus Alteromonas by PFGE.
对耐三丁基锡海洋细菌M-1菌株的16S rRNA进行了部分测序，以确认其分类地位。结果表明，该细菌应归类于交替单胞菌属，而非之前报道中所鉴定的弧菌属。还使用轮廓钳位均匀电场脉冲场凝胶电泳（PFGE）测量了该菌株的基因组大小。发现该菌株的基因组大小为2240千碱基对，而黑变交替单胞菌和腐败希瓦氏菌的基因组大小分别为2040千碱基对和2383千碱基对。这是首次通过PFGE对交替单胞菌属进行基因组大小测定的报告。
