Hewett S J, Corbett J A, McDaniel M L, Choi D W
Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
Neurosci Lett. 1993 Dec 24;164(1-2):229-32. doi: 10.1016/0304-3940(93)90898-u.
An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1 beta (IL1 beta) plus interferon-gamma (INF gamma) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor NG-nitro-L-arginine and the protein synthesis inhibitor cycloheximide.
在某些细胞类型中存在一种能够产生大量一氧化氮（NO）的诱导型一氧化氮合酶（iNOS）。我们通过免疫沉淀和亚硝酸盐形成证明，白细胞介素-1β（IL1β）加干扰素-γ（INFγ）可诱导小鼠皮质星形胶质细胞原代培养物中一氧化氮合酶的表达。这种诱导具有时间和剂量依赖性，并受到一氧化氮合酶抑制剂NG-硝基-L-精氨酸和蛋白质合成抑制剂环己酰亚胺的抑制。
