Kobayashi H, Espinosa R, Fernald A A, Begy C, Diaz M O, Le Beau M M, Rowley J D
Department of Medicine, University of Chicago, IL 60637.
Genes Chromosomes Cancer. 1993 Dec;8(4):246-52. doi: 10.1002/gcc.2870080407.
We studied samples containing deletions of the long arm of chromosome 11 (11q) from patients with hematologic malignancies by using cytogenetic and fluorescence in situ hybridization (FISH) techniques. Cytogenetic analysis of 28 patients and of a cell line showed that all deletions included band 11q23. FISH analysis demonstrated that the proximal part of 11q23, including NCAM, was deleted in 13 of 15 patients and the cell line. Recurring chromosomal losses in human tumors have been regarded as evidence that the affected regions contain tumor-suppressor genes. These results suggest that the putative tumor-suppressor gene is proximal to the MLL gene which is also located in 11q23.
我们运用细胞遗传学和荧光原位杂交（FISH）技术，对血液系统恶性肿瘤患者中含有11号染色体长臂（11q）缺失的样本进行了研究。对28例患者和1个细胞系进行的细胞遗传学分析显示，所有缺失均包含11q23带。FISH分析表明，在15例患者中的13例以及该细胞系中，11q23的近端部分（包括NCAM）发生了缺失。人类肿瘤中反复出现的染色体缺失被视为受影响区域含有肿瘤抑制基因的证据。这些结果表明，假定的肿瘤抑制基因位于同样定位于11q23的MLL基因的近端。
