甲醇醋杆菌MB 70的荚膜多糖和脂多糖O侧链的结构，以及由噬菌体Acm6降解产生的寡糖的结构。

Structure of the capsular polysaccharide and the O-side-chain of the lipopolysaccharide from Acetobacter methanolicus MB 70, and of oligosaccharides resulting from their degradation by the bacteriophage Acm6.

作者信息

Grimmecke H D, Knirel Y A, Shashkov A S, Kiesel B, Lauk W, Voges M

机构信息

Institute of Biotechnology, Leipzig, Germany.

出版信息

Carbohydr Res. 1994 Feb 3;253:277-82. doi: 10.1016/0008-6215(94)80072-3.

DOI:10.1016/0008-6215(94)80072-3

PMID:7512445

Abstract

Acetobacter methanolicus MB 70 was shown to be related to the type strain of this species MB 58/4 (IMET 10945) having the same galactan-->2)-beta-D-Gal f-(1-->3)-beta-D-Gal p-(1-->as the capsular polysaccharide (CPS) and the O-side-chain of the lipopolysaccharide (LPS). Additionally, a glucan built up of the disaccharide repeating unit-->6)-alpha-D-Glc p-(1-->2)-alpha-D-Glc p-(1-->was identified in strain MB 70. In the CPS, the polymers were present in the ratio approximately 1:1, whereas the glucan preponderated in the LPS. Bacteriophage Acm6 specific to A. methanolicus MB 70 hydrolysed selectively the glucan component of both CPS and LPS. Structural elucidation of the resulting oligosaccharides led to the identification of the phage-associated depolymerase as an endo-alpha-(1-->6)-D-glucopyranoside hydrolase.

摘要

甲醇醋杆菌MB 70被证明与该物种的模式菌株MB 58/4（IMET 10945）相关，它们具有相同的半乳聚糖-->2)-β-D-半乳呋喃糖-(1-->3)-β-D-吡喃半乳糖-(1-->作为荚膜多糖（CPS）和脂多糖（LPS）的O-侧链。此外，在菌株MB 70中鉴定出一种由二糖重复单元-->6)-α-D-吡喃葡萄糖-(1-->2)-α-D-吡喃葡萄糖-(1-->构成的葡聚糖。在CPS中，这两种聚合物的比例约为1:1，而葡聚糖在LPS中占优势。特异性针对甲醇醋杆菌MB 70的噬菌体Acm6选择性地水解CPS和LPS的葡聚糖成分。对所得寡糖的结构解析导致鉴定出噬菌体相关的 depolymerase 为内切-α-(1-->6)-D-葡萄糖苷水解酶。