Hormia M, Könönen M
Department of Periodontology, University of Helsinki, Finland.
J Periodontal Res. 1994 Mar;29(2):146-52. doi: 10.1111/j.1600-0765.1994.tb01103.x.
The adhesion and spreading of human gingival fibroblasts on glass and differently processed titanium surfaces was studied by immunolocalization of vinculin and the alpha and beta subunits of the fibronectin (alpha 5 beta 1) and (alpha v beta 3) receptors. Vinculin-containing focal contacts were present both at 4 and 24 h of spreading in cells grown on glass or electropolished or etched titanium surfaces but not in cells spreading on sandblasted titanium surfaces. Immunostaining for the alpha 5 and beta 1 subunits of the fibronectin receptor showed only a diffuse membrane fluorescence after 4 h of cell spreading irrespective of the growth surface. The alpha v and beta 3 subunits of the vitronectin receptor were at this stage detected in focal contacts in cells spreading on glass or electropolished or etched titanium surfaces. In cells spreading on sandblasted titanium surfaces, however, the vitronectin receptor had only a diffuse distribution. In cells that had been allowed to spread for 24 h on glass or electropolished or etched titanium surfaces the alpha 5 and beta 1 integrin subunits were either diffusely distributed or showed a localization typical of extracellular matrix contacts. The alpha v and beta 3 integrin subunits were, as earlier, localized to typical focal contacts in cells grown on glass or electropolished or etched titanium surfaces. Cells attached to sandblasted titanium surfaces still expressed all the integrin subunits only diffusely. The results show that the surface texture of the substratum can affect the expression of integrin subunits in human gingival fibroblasts.(ABSTRACT TRUNCATED AT 250 WORDS)
通过对纽蛋白以及纤连蛋白（α5β1）和（αvβ3）受体的α和β亚基进行免疫定位，研究了人牙龈成纤维细胞在玻璃和不同处理的钛表面上的黏附与铺展情况。在玻璃、电解抛光或蚀刻钛表面生长的细胞铺展4小时和24小时时均存在含纽蛋白的黏着斑，但在喷砂处理的钛表面铺展的细胞中则没有。无论生长表面如何，细胞铺展4小时后，纤连蛋白受体α5和β1亚基的免疫染色仅显示弥漫性膜荧光。在此阶段，在玻璃、电解抛光或蚀刻钛表面铺展的细胞的黏着斑中检测到了玻连蛋白受体的αv和β3亚基。然而，在喷砂处理的钛表面铺展的细胞中，玻连蛋白受体仅呈弥漫性分布。在玻璃、电解抛光或蚀刻钛表面铺展24小时的细胞中，α5和β1整合素亚基要么呈弥漫性分布，要么显示出细胞外基质接触的典型定位。αv和β3整合素亚基与之前一样，定位于在玻璃、电解抛光或蚀刻钛表面生长的细胞中的典型黏着斑处。附着在喷砂处理的钛表面的细胞仍然仅弥漫性表达所有整合素亚基。结果表明，基质的表面质地会影响人牙龈成纤维细胞中整合素亚基的表达。（摘要截短至250字）
