用人类玻连蛋白探针鉴定出的麝香百合中的两类蛋白质和信使核糖核酸。
Two classes of proteins and mRNAs in Lilium longiflorum L. indentified by human vitronectin probes.
作者信息
Wang C S, Walling L L, Gu Y Q, Ware C F, Lord E M
机构信息
Department of Botany and Plant Sciences, University of California, Riverside 92521-0124.
出版信息
Plant Physiol. 1994 Feb;104(2):711-7. doi: 10.1104/pp.104.2.711.
Vitronectin (VN) is a substrate adhesion molecule, an extracellular matrix glycoprotein that facilitates cell adhesion and cell movement in animals. We have reported the cross-reactivity of a 55-kD protein in plants with rabbit anti-human VN antibodies and the presence of VN-like sequences in plant genomes using a human VN cDNA probe. We have extended these studies by using human VN riboprobes to detect VN-like mRNAs in lily (Lilium longiflorum L.) and soybean. In both species, two mRNAs were detected. We have also identified a new cross-reactive protein (41 kD) using a different preparation of human VN antiserum. In lily roots five 41-kD isoforms were observed, whereas only three of these isoforms accumulated in leaves. Monospecific antibodies prepared against the plant proteins cross-reacted with the human VN protein and vice versa. We have purified the 41-kD protein using two-dimensional gel electrophoresis, and amino acid composition analysis indicates that it is similar in composition to human VN.
玻连蛋白（VN）是一种底物粘附分子，是一种细胞外基质糖蛋白，可促进动物体内的细胞粘附和细胞运动。我们已经报道了植物中一种55-kD蛋白与兔抗人VN抗体的交叉反应性，以及使用人VN cDNA探针在植物基因组中存在VN样序列。我们通过使用人VN核糖探针来检测百合（Lilium longiflorum L.）和大豆中的VN样mRNA，扩展了这些研究。在这两个物种中，均检测到两种mRNA。我们还使用不同制备的人VN抗血清鉴定出一种新的交叉反应蛋白（41 kD）。在百合根中观察到五种41-kD同工型，而这些同工型中只有三种在叶片中积累。针对植物蛋白制备的单特异性抗体与人类VN蛋白发生交叉反应，反之亦然。我们使用二维凝胶电泳纯化了41-kD蛋白，氨基酸组成分析表明其组成与人类VN相似。
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