Ohnishi T, Nakamura O, Arakaki N, Miyazaki H, Daikuhara Y
Department of Biochemistry, Kagoshima University Dental School, Japan.
Biochem Biophys Res Commun. 1994 Apr 15;200(1):598-605. doi: 10.1006/bbrc.1994.1490.
Recently, we showed that a 59 kDa non-phosphorylated sialoprotein purified from rat bone matrix is the rat counterpart of bovine fetuin and human alpha 2-HS glycoprotein and that fetuin synthesized and secreted by adult rat hepatocytes in primary culture is mostly phosphorylated (phosphofetuin), though fetuin is known to contain no phosphorus. Here we report that the rate of synthesis of phosphofetuin by hepatocytes in culture was reduced by inflammatory cytokines such as human interleukin (hIL)-6, human tumor necrosis factor-alpha and hIL-1 alpha, but dose-dependently stimulated by growth factors of hepatocytes, such as hepatocyte growth factor (HGF)/scatter factor (SF), epidermal growth factor and insulin, as determined by metabolic labeling and Northern blot analysis using cDNA for rat fetuin as a probe. We also showed that administration of HGF/SF stimulated gene expression of rat fetuin in vivo.
最近，我们发现从大鼠骨基质中纯化出的一种59 kDa非磷酸化唾液酸糖蛋白是牛胎球蛋白和人α2-HS糖蛋白的大鼠对应物，并且原代培养的成年大鼠肝细胞合成并分泌的胎球蛋白大多是磷酸化的（磷酸胎球蛋白），尽管已知胎球蛋白不含磷。在此我们报告，培养的肝细胞合成磷酸胎球蛋白的速率受到人白细胞介素（hIL）-6、人肿瘤坏死因子-α和hIL-1α等炎性细胞因子的抑制，但受到肝细胞生长因子如肝细胞生长因子（HGF）/分散因子（SF）、表皮生长因子和胰岛素的剂量依赖性刺激，这是通过代谢标记和使用大鼠胎球蛋白cDNA作为探针的Northern印迹分析确定的。我们还表明，给予HGF/SF可在体内刺激大鼠胎球蛋白的基因表达。
