Dulhanty A M, Riordan J R
Cystic Fibrosis Research Development Program, Hospital for Sick Children, Toronto, Ont., Canada.
FEBS Lett. 1994 Apr 25;343(2):109-14. doi: 10.1016/0014-5793(94)80300-5.
CFTR belongs to a group of proteins sharing the structural motif of six transmembrane helices and a nucleotide binding domain. Unique to CFTR is the R domain, a charged cytoplasmic domain. Comparison of R domain sequences from ten species revealed that the N-terminal third is highly conserved, while the C-terminal two-thirds is poorly conserved. The R domain shows no strong sequence similarity to known proteins; however, 14 viral pol proteins show limited similarity to fragments of the R domain. Analysis revealed a relationship between the N- and C-terminal fragments of the R domain and two discontinuous fragments of the pol protein. These observations support a two-domain model for the R domain.
囊性纤维化跨膜传导调节因子（CFTR）属于一组具有六个跨膜螺旋结构基序和一个核苷酸结合域的蛋白质。CFTR特有的是R结构域，一个带电荷的胞质结构域。对来自十个物种的R结构域序列进行比较发现，其N端三分之一高度保守，而C端三分之二保守性较差。R结构域与已知蛋白质没有很强的序列相似性；然而，14种病毒多聚蛋白与R结构域的片段有有限的相似性。分析揭示了R结构域的N端和C端片段与多聚蛋白的两个不连续片段之间的关系。这些观察结果支持了R结构域的双结构域模型。
