Tanaka S, Koike T
Department of Natural Science, Saga Medical School, Japan.
Neuropeptides. 1994 Feb;26(2):103-11. doi: 10.1016/0143-4179(94)90101-5.
Neuropeptides were examined for their effects on the survival of cultured rat superior cervical ganglion cells after acute deprivation of nerve growth factor (NGF). Vasoactive intestinal peptide (VIP, 3 microM) delayed the neuronal death about 6 h alone. The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (0.2 mM) greatly potentiated its effect, reducing EC50 from 2.5 microM to 8 nM. The neuronal death was completely suppressed under this condition. On the other hand, substance P (1-100 microM) or enkephalin (1-100 microM) alone did not modify the death, whereas the latter (100 microM) enhanced the survival-promoting effect of membrane depolarization with elevated K+. These results suggest strongly that neuropeptides regulate the NGF-independent survival of sympathetic neurons through a cAMP-dependent mechanism.
研究了神经肽对急性剥夺神经生长因子（NGF）后培养的大鼠颈上神经节细胞存活的影响。血管活性肠肽（VIP，3 microM）单独作用时可使神经元死亡延迟约6小时。磷酸二酯酶抑制剂3 - 异丁基 - 1 - 甲基黄嘌呤（0.2 mM）极大地增强了其作用，将半数有效浓度（EC50）从2.5 microM降至8 nM。在此条件下，神经元死亡被完全抑制。另一方面，单独的P物质（1 - 100 microM）或脑啡肽（1 - 100 microM）并未改变细胞死亡情况，而后者（100 microM）增强了高钾引起的膜去极化的促存活作用。这些结果有力地表明，神经肽通过一种依赖环磷酸腺苷（cAMP）的机制调节交感神经元不依赖NGF的存活。
