Cox A L, Skipper J, Chen Y, Henderson R A, Darrow T L, Shabanowitz J, Engelhard V H, Hunt D F, Slingluff C L
Department of Chemistry, University of Virginia, Charlottesville 22908.
Science. 1994 Apr 29;264(5159):716-9. doi: 10.1126/science.7513441.
Of several thousand peptides presented by the major histocompatibility molecule HLA-A2.1, at least nine are recognized by melanoma-specific cytotoxic T lymphocytes (CTLs). Tandem mass spectrometry was used to identify and to sequence one of these peptide epitopes. Melanoma-specific CTLs had an exceptionally high affinity for this nine-residue peptide, which reconstituted an epitope for CTL lines from each of five different melanoma patients tested. Recognition by multiple CTL lines suggests that this may be a promising candidate for use in peptide-based melanoma vaccines.
在主要组织相容性分子HLA - A2.1呈递的数千种肽中，至少有九种能被黑色素瘤特异性细胞毒性T淋巴细胞（CTL）识别。串联质谱法被用于鉴定和测序其中一个肽表位。黑色素瘤特异性CTL对这种九肽具有异常高的亲和力，该九肽为所测试的五名不同黑色素瘤患者的CTL系重建了一个表位。多个CTL系的识别表明，这可能是基于肽的黑色素瘤疫苗的一个有前景的候选物。
