激动素处理的烟草愈伤组织中苯丙氨酸解氨酶转录本的短暂增加。
A transient increase of phenylalanine ammonia-lyase transcript in kinetin-treated tobacco callus.
作者信息
Nagai N, Kitauchi F, Okamoto K, Kanda T, Shimosaka M, Okazaki M
机构信息
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
出版信息
Biosci Biotechnol Biochem. 1994 Mar;58(3):558-9. doi: 10.1271/bbb.58.558.
In tobacco cell culture (Nicotiana tabacum L. "Bright Yellow" T-13), phenylalanine ammonia-lyase (PAL) activity was induced in response to an exogenously added kinetin. RNA blot hybridization analysis showed that a single species of PAL transcript 2.9-kb in size was detected using the PAL cDNA cloned from kinetin-treated cells. The cellular content of this transcript increased transiently (2.5-fold) 4 h after the addition of kinetin followed by an increase in PAL enzyme activity at 16 h.
在烟草细胞培养（烟草“亮黄”T-13）中，苯丙氨酸解氨酶（PAL）活性因外源添加激动素而被诱导。RNA印迹杂交分析表明，使用从经激动素处理的细胞中克隆的PAL cDNA，检测到一种大小为2.9 kb的单一PAL转录本。添加激动素4小时后，该转录本的细胞含量短暂增加（2.5倍），随后在16小时时PAL酶活性增加。
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