Chen W, Qiu L S
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1993;11(2):135-7.
A rapid method of total RNA of Schistosoma japonicum adult worm isolation by single step extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of intact RNA and can be completed within several hours. It is particularly useful for isolation of RNA from minute quantity of parasite material. Analysis of agarose gels electrophoresis reveals that the RNA preparations show a distinct band comigrating with 18S rat brain RNA and without large rRNA species. The large rRNA is in vivo nicked. The absence of a band at approximately 26S presumably reflects post transcriptional processing of the large rRNA.
描述了一种用酸性异硫氰酸胍 - 苯酚 - 氯仿混合物一步提取法快速分离日本血吸虫成虫总RNA的方法。该方法可提供完整RNA的纯制剂，且能在数小时内完成。它对于从微量寄生虫材料中分离RNA特别有用。琼脂糖凝胶电泳分析表明，RNA制剂显示出与18S大鼠脑RNA共迁移的清晰条带，且没有大的rRNA种类。大的rRNA在体内有切口。大约26S处没有条带可能反映了大rRNA的转录后加工。
