Lefer A M, Ma X L
Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-6799.
J Appl Physiol (1985). 1994 Jan;76(1):33-8. doi: 10.1152/jappl.1994.76.1.33.
We studied polymorphonuclear leukocyte (PMN) adherence to cat ischemic-reperfused mesenteric artery and vein endothelia under conditions of flow in vitro. Under physiological shear rates, only a few PMNs adhered to non-ischemic-reperfused arterial and venous endothelia (11 +/- 2 and 20 +/- 3 PMN/mm2, respectively). However, after 60 min of ischemia and 20 or 120 min of reperfusion, a significant increase in PMN adherence to arterial endothelium was observed. At 20 min of reperfusion, 44 +/- 4 PMN/mm2 adhered, and at 120 min postreperfusion, 63 +/- 12 PMN/mm2 adhered (P < 0.01 from control). Moreover, a greater degree of PMN adherence occurred on the venous than on the arterial endothelium. Thus, 159 +/- 10 and 198 +/- 12 PMN/mm2 adhered to mesenteric venous endothelium isolated after 20 and 120 min reperfusion, respectively (P < 0.01 vs. arteries). Furthermore, addition of PB 1.3 (20 micrograms/ml), a monoclonal antibody against P-selectin, 5 min before perfusion with PMNs significantly attenuated the increase in PMN adherence to both arterial and venous endothelia (P < 0.01). These results indicate that PMN-endothelial interaction also occurs in conduit vessels after ischemia and reperfusion, although a more profound PMN adherence occurs in veins.
我们在体外流动条件下研究了多形核白细胞（PMN）对猫缺血再灌注肠系膜动脉和静脉内皮的黏附情况。在生理剪切速率下，只有少数PMN黏附于非缺血再灌注的动脉和静脉内皮（分别为11±2和20±3个PMN/mm²）。然而，在缺血60分钟以及再灌注20或120分钟后，观察到PMN对动脉内皮的黏附显著增加。再灌注20分钟时，有44±4个PMN/mm²黏附，再灌注120分钟时，有63±12个PMN/mm²黏附（与对照组相比，P<0.01）。此外，PMN在静脉内皮上的黏附程度高于动脉内皮。因此，再灌注20分钟和120分钟后分离出的肠系膜静脉内皮上分别有159±10和198±12个PMN/mm²黏附（与动脉相比，P<0.01）。此外，在PMN灌注前5分钟加入抗P选择素单克隆抗体PB 1.3（20微克/毫升）可显著减弱PMN对动脉和静脉内皮黏附的增加（P<0.01）。这些结果表明，缺血再灌注后在导管血管中也会发生PMN与内皮的相互作用，尽管PMN在静脉中的黏附更为明显。
