Kennerley G A, Parry J M
School of Biological Sciences, University of Wales, Swansea, Singleton Park, UK.
Mutat Res. 1994 May 1;307(1):223-8. doi: 10.1016/0027-5107(94)90295-x.
Larval and toadlet stages of the clawed toad Xenopus laevis were exposed to benzo[a]pyrene in aquatic media. Mutations were analysed in a variety of restriction enzyme recognition sequences of the adult alpha 1-globin gene. Mutations were detected in the BsiLi recognition site (CCTGG) at early sampling times (6 h) in larval stages and at 8-days sampling time in post-metamorphosis toadlets. The predominant mutation detected was a G-->T base transversion at the 5th base of the CCTGG sequence. The data presented indicates that the Restriction-Site Mutation methodology has considerable potential for development as a technique for monitoring the genotoxic potential of water-borne toxins.
将非洲爪蟾非洲爪蟾的幼体和蟾蜍幼体阶段暴露于水生介质中的苯并[a]芘中。对成年α1-珠蛋白基因的多种限制性内切酶识别序列中的突变进行了分析。在幼体阶段的早期采样时间（6小时）以及变态后蟾蜍幼体的8天采样时间，在BsiLi识别位点（CCTGG）检测到了突变。检测到的主要突变是CCTGG序列第5个碱基处的G→T碱基颠换。所呈现的数据表明，限制性位点突变方法作为一种监测水中毒素遗传毒性潜力的技术具有相当大的发展潜力。
