Allan G L, Camakaris J, Legge G J
Micro Analytical Research Centre, School of Physics, University of Melbourne, Parkville, Victoria, Australia.
Biol Trace Elem Res. 1994 Feb;40(2):103-26. doi: 10.1007/BF02950785.
A scanning proton microprobe has been used for the elemental microanalysis of individual fibroblast cells. Both normal fibroblasts and fibroblasts cultured from patients with Menkes' disease, an X-linked genetic disorder known to be associated with defective copper metabolism, were examined by the probe. The cells were cultured on a thin ultra-clean nylon foil and retained on that surface for analysis. The focused high-energy proton beam was used to irradiate selected individual cells and elemental information was derived from X-ray and backscattered proton data. The sensitivity of the scanning proton microprobe to trace concentrations of heavy elements has allowed this elemental information to be used to identify individual cells as being either normal or a Menkes' mutant. The cell identification was based on the application of discriminate analysis to a data set formed from the ratios of copper to each of the macroelements present in the cell. This method of cell identification offers the promise of rapid diagnosis of Menkes' disease.
扫描质子微探针已用于单个成纤维细胞的元素微分析。该探针检测了正常成纤维细胞以及从患有门克斯病（一种已知与铜代谢缺陷相关的X连锁遗传病）患者身上培养的成纤维细胞。细胞在超薄超净尼龙箔上培养，并保留在该表面进行分析。聚焦的高能质子束用于照射选定的单个细胞，元素信息来自X射线和背散射质子数据。扫描质子微探针对痕量重金属元素浓度的敏感性使得该元素信息可用于识别单个细胞是正常细胞还是门克斯突变细胞。细胞识别基于对由细胞中铜与每种大量元素的比率形成的数据集应用判别分析。这种细胞识别方法有望实现门克斯病的快速诊断。
