Kulakauskas S, Barsomian J M, Lubys A, Roberts R J, Wilson G G
Cold Spring Harbor Laboratory, NY 11724.
Gene. 1994 May 3;142(1):9-15. doi: 10.1016/0378-1119(94)90348-4.
We report the organization of the HpaII restriction and modification (R-M) system from Haemophilus parainfluenzae (recognition sequence: 5'...CCGG...3'), the sequence of the gene coding for the HpaII restriction endonuclease, and the sequence of the upstream flanking DNA. The HpaII system comprises two genes, hpaIIM, coding for the methyltransferase (MTase; 358 amino acids (aa), 40.4 kDa: product, Cm5CGG), and hpaIIR, coding for the restriction endonuclease (ENase; 358 aa, 40.9 kDa: product, C'CGG). The genes are adjacent, they have the same orientation, and they occur in the order hpaIIM then hpaIIR. The ENase bears little as sequence similarity to the isoschizomeric R.BsuFI and R.MspI ENases. Upstream of, and partly overlapping hpaIIM is the coding sequence for a 141-aa protein that resembles the very-short-patch-repair endonuclease (Vsr) of Escherichia coli. Upstream of that is the coding sequence for a protein that resembles valyl-tRNA synthetase (ValS).
我们报道了副流感嗜血杆菌HpaII限制与修饰（R-M）系统的组织情况（识别序列：5'...CCGG...3'）、编码HpaII限制内切酶的基因序列以及上游侧翼DNA序列。HpaII系统由两个基因组成，hpaIIM编码甲基转移酶（MTase；358个氨基酸（aa），40.4 kDa：产物，Cm5CGG），hpaIIR编码限制内切酶（ENase；358 aa，40.9 kDa：产物，C'CGG）。这些基因相邻，方向相同，且按hpaIIM、hpaIIR的顺序排列。该内切酶与同裂酶R.BsuFI和R.MspI内切酶的序列相似性很低。在hpaIIM上游且部分与之重叠的是一个编码141个氨基酸蛋白质的序列，该蛋白质类似于大肠杆菌的极短补丁修复内切酶（Vsr）。在其上游是一个编码类似于缬氨酰-tRNA合成酶（ValS）蛋白质的序列。
