Asano H, Hotta T, Ichihara M, Murate T, Kobayashi M, Saito H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Leukemia. 1994 May;8(5):833-8.
The in vitro colony-forming capacities of marrow CD34-positive (CD34+) cells from 25 patients with myelodysplastic syndromes (MDS) were studied. In all patients this purified cell population showed erythroid (burst-forming unit erythroid; BFU-E) or non-erythroid colony growth, both of which were more restricted than non-purified mononuclear cell population under stimulation with erythropoietin (EPO) or granulocyte/macrophage colony-stimulating factor (GM-CSF) alone. MDS patients examined were put into two groups; refractory anemia (RA) or RA with ringed sideroblasts (RA/RARS) and RA with excess of blasts (RAEB) or RAEB in transformation (RAEB/RAEB-t). The CD34+ cells of both RA/RARS and RAEB/RAEB-t exhibited a similarity to the cells of normal individuals in their responsiveness to the addition of interleukin 6 (IL-6), IL-3 or stem cell factor (SCF). SCF caused powerful but highly variable responses in both erythroid and nonerythroid colony formation as compared with IL-6 or IL-3. We demonstrated that MDS marrow hematopoietic progenitor cells reactive to GM-CSF or EPO were additionally stimulated with early-acting hematopoietic growth factors including IL-6, IL-3 and SCF in a fashion similar to those of normal individuals.
对25例骨髓增生异常综合征（MDS）患者的骨髓CD34阳性（CD34+）细胞的体外集落形成能力进行了研究。在所有患者中，这种纯化的细胞群体均显示出红系（红系爆式集落形成单位；BFU-E）或非红系集落生长，在单独使用促红细胞生成素（EPO）或粒细胞/巨噬细胞集落刺激因子（GM-CSF）刺激下，这两种集落生长均比未纯化的单核细胞群体受到更多限制。所检查的MDS患者分为两组；难治性贫血（RA）或伴有环形铁粒幼细胞的RA（RA/RARS）以及伴有原始细胞增多的RA（RAEB）或转化中的RAEB（RAEB/RAEB-t）。RA/RARS和RAEB/RAEB-t的CD34+细胞在对添加白细胞介素6（IL-6）、IL-3或干细胞因子（SCF）的反应性方面与正常个体的细胞表现出相似性。与IL-6或IL-3相比，SCF在红系和非红系集落形成中均引起强烈但高度可变的反应。我们证明，对GM-CSF或EPO有反应的MDS骨髓造血祖细胞，会受到包括IL-6、IL-3和SCF在内的早期作用造血生长因子的额外刺激，其方式与正常个体相似。
