Immunoreactivity for diazepam binding inhibitor in Gomori-positive astrocytes.

A polypeptide termed diazepam binding inhibitor (DBI), capable of binding to receptor sites on glial mitochondria, is known to be present in glial cells and is particularly abundant in areas near circumventricular organs of the brain such as the arcuate nucleus of the hypothalamus. DBI appears to stimulate steroid synthesis and/or transport in glial mitochondria. The arcuate nucleus also contains large numbers of specialized glia, termed Gomori-positive astrocytes, that are estrogen-sensitive and which possess highly stained, heme-containing cytoplasmic granules. This study was performed to determine if these Gomori-positive astrocytes are immunoreactive for DBI. A rabbit antibody to DBI, but not pre-immune serum, stained Gomori-positive glia and suggests that these glia are partly responsible for the high levels of DBI in circumventricular organs. DBI in these glia may be related to functional responses of the hypothalamus to steroid hormones.