Morphology of two classes of target-specific bullfrog sympathetic preganglionic neurons.

These experiments took advantage of the unique ability to define target-specific sympathetic preganglionic neurons in the bullfrog spinal cord in order to examine the morphologies of different classes of preganglionic neurons. Sympathetic preganglionic neurons were identified by retrograde transport of fast blue from the sympathetic chain. Subsequently, fast blue-labelled sympathetic preganglionic neurons in fixed spinal cord slices were filled with lucifer yellow and processed for visualization with lucifer yellow antiserum, biotinylated secondary antiserum, and avidin peroxidase. Target specificity of sympathetic preganglionic neurons was determined by anatomical position; sympathetic preganglionic neurons that control the vasculature (C-type sympathetic preganglionic neurons) lie in a position caudal to those that control nonvascular targets [B-type sympathetic preganglionic neurons; Horn and Stofer (1988) J. Comp. Neurol. 268:71]. These two classes of sympathetic preganglionic neurons have qualitatively similar morphologies. However, they exhibit significant quantitative differences in total dendritic length and the rostrocaudal extent of dendrites. These differences are likely to be associated with differences in the number of synapses received by these two classes of sympathetic preganglionic neurons. Moreover, the segmental control of sympathetic preganglionic neurons by descending brainstem projections is likely to be finer for those involved in vascular control than for those that influence other targets.