Martín-Orozco E, Cárdaba B, del Pozo V, de Andrés B, Villalba M, Gallardo S, Rodriguez-García M I, Fernández M C, Alché J D, Rodriguez R
Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.
Int Arch Allergy Immunol. 1994;104(2):160-70. doi: 10.1159/000236725.
Ole e I is the major allergen derived from olive tree pollen (Olea europaea) and it is composed of two polypeptides with molecular weights (MWs) of 18 and 20 kD. A panel of six monoclonal antibodies (mAbs) has been prepared and used to map antigenic determinants on this molecule. Four epitope determinants have been identified on Ole e I. Using the purified mAbs produced against Ole e I, we have analyzed the common epitope determinants in olive (O. europaea) and different Oleaceae pollens: ash (Fraxinus excelsior); privet (Ligustrum vulgare); lilac (Syringa vulgaris), and forsythia (Forsythia suspensa). ELISA showed three reactivity groups depending on the recognition of monoclonal antibodies: (1) olive and ash; (2) olive, ash, privet and lilac; and (3) olive, ash, privet, lilac and forsythia. Immunoblotting studies on Oleaceae pollen extracts with these mAbs showed a very similar cross-reactivity pattern. The 18- and 20-kD MW proteins were present in each pollen, except in the case of forsythia. In this case the reactivity pattern was associated with 50- to 55-kD protein bands. This band was recognized by a pool of sera from olive-allergic patients. Finally, ultrastructural localization of Ole e I antigen was performed on the mature olive pollen grain. Ole e I was located in association with dilated endoplasmic reticulum cisternae. Pollen grain walls, nuclei and cytoplasmic organelles were totally devoid of the allergen.
油橄榄I是从油橄榄树花粉（油橄榄）中提取的主要过敏原，它由两种分子量分别为18kD和20kD的多肽组成。已制备了一组六种单克隆抗体（mAb），并用于绘制该分子上的抗原决定簇图谱。在油橄榄I上已鉴定出四个表位决定簇。使用针对油橄榄I产生的纯化单克隆抗体，我们分析了油橄榄（油橄榄）和不同木犀科花粉中的共同表位决定簇：白蜡树（欧洲白蜡树）；女贞（欧洲女贞）；丁香（紫丁香）和连翘（连翘）。酶联免疫吸附测定（ELISA）显示，根据单克隆抗体的识别情况可分为三个反应组：（1）油橄榄和白蜡树；（2）油橄榄、白蜡树、女贞和丁香；（3）油橄榄、白蜡树、女贞、丁香和连翘。用这些单克隆抗体对木犀科花粉提取物进行的免疫印迹研究显示出非常相似的交叉反应模式。除连翘外，每种花粉中都存在分子量为18kD和20kD的蛋白质。在这种情况下，反应模式与50至55kD的蛋白带相关。该条带可被油橄榄过敏患者的一组血清识别。最后，对成熟油橄榄花粉粒进行了油橄榄I抗原的超微结构定位。油橄榄I定位于扩张的内质网池附近。花粉粒壁、细胞核和细胞质细胞器完全没有该过敏原。
