Pace L W, Kreeger J M, Miller M A, Turk J R, Fischer J R
Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia.
Vet Pathol. 1994 Mar;31(2):168-72. doi: 10.1177/030098589403100202.
Malignant fibrous histiocytoma was diagnosed in seven cats from biopsy specimens received at the University of Missouri Veterinary Medical Diagnostic Laboratory during a 4-year period from 1987-1991. Tissue blocks from formalin-fixed specimens were resectioned and stained for type I (AE1) and type II (AE3) cytokeratins, desmin, S100 protein, vimentin, and alpha 1-antitrypsin by the avidin biotin peroxidase complex method with diaminobenzidine (DAB) chromogen. None of the tumors stained positively for alpha 1-antitrypsin. Four of seven of the tumors had similar immunohistochemical staining results, with positive staining for type I and type II cytokeratins, desmin, S100 protein, and vimentin. Of the remaining three, one stained positively only for S100 protein and vimentin; one stained positively for vimentin only; and one was negative for all six antigens. Based only on immunohistochemical staining results, three of the tumors could possibly be reclassified: one as a melanoma, one as a probable fibrosarcoma, and one as unknown. These results also indicate that feline malignant fibrous histiocytomas show a diversity of intermediate filament expression, as do human tumors. Our results also do not support the theory that malignant fibrous histiocytomas are of histiocytic origin.
1987年至1991年的4年间，密苏里大学兽医医学诊断实验室从活检标本中诊断出7只猫患有恶性纤维组织细胞瘤。对福尔马林固定标本的组织块进行重新切片，采用抗生物素蛋白生物素过氧化物酶复合物法，以二氨基联苯胺（DAB）显色剂对I型（AE1）和II型（AE3）细胞角蛋白、结蛋白、S100蛋白、波形蛋白和α1-抗胰蛋白酶进行染色。所有肿瘤的α1-抗胰蛋白酶染色均为阴性。7个肿瘤中有4个具有相似的免疫组化染色结果，I型和II型细胞角蛋白、结蛋白、S100蛋白和波形蛋白染色呈阳性。其余3个肿瘤中，1个仅S100蛋白和波形蛋白染色呈阳性；1个仅波形蛋白染色呈阳性；1个对所有6种抗原均呈阴性。仅根据免疫组化染色结果，3个肿瘤可能需要重新分类：1个为黑色素瘤，1个可能为纤维肉瘤，1个分类不明。这些结果还表明，猫恶性纤维组织细胞瘤与人类肿瘤一样，显示出中间丝表达的多样性。我们的结果也不支持恶性纤维组织细胞瘤起源于组织细胞的理论。
