Thoolen R J, Vos J H, van der Linde-Sipman J S, de Weger R A, van Unnik J A, Misdorp W, van Dijk J E
Department of Veterinary Pathology, State University, Utrecht, The Netherlands.
Res Vet Sci. 1992 Sep;53(2):198-204. doi: 10.1016/0034-5288(92)90110-n.
Immunohistochemical staining was performed on seven canine and 10 feline soft tissue tumours histologically diagnosed as malignant fibrous histiocytomas (MFHs) or MFH-like tumours, and eight other histologically specified tumours (non-MFH). This was done to determine if commercially available antibodies that are used routinely in human diagnostic pathology for MFHs would express the same immunohistochemical patterns in canine and feline MFHs and MFH-like tumours. The antibodies were directed against human alpha 1-anti-trypsin (AT), human alpha 1-anti-chymotrypsin (ACT), human lysozyme, bovine S-100 protein and human desmin. AT did not show any immunoreactivity in the tissues investigated. Except for one MFH, all canine MFHs and other soft tissue tumours with a 'histiocytic' character stained for lysozyme and not for S-100. Six out of seven canine MFHs and MFH-like tumours stained positive for desmin as did most non-MFH sarcomas. Most of the canine and feline MFHs and MFH-like tumours were positive for ACT. These findings for ACT staining in canine and feline MFHs and MFH-like tumours are in agreement with the findings in human MFHs. The immunohistochemical results of canine MFHs and MFH-like tumours were different from those in cats. Feline MFHs differed from canine MFHs for both lysozyme and desmin staining.
对7例犬类和10例猫类的软组织肿瘤进行了免疫组织化学染色，这些肿瘤经组织学诊断为恶性纤维组织细胞瘤（MFH）或MFH样肿瘤，以及8例其他组织学明确的肿瘤（非MFH）。这样做是为了确定在人类诊断病理学中常规用于MFH的市售抗体在犬类和猫类MFH及MFH样肿瘤中是否会表现出相同的免疫组织化学模式。这些抗体针对人α1 -抗胰蛋白酶（AT）、人α1 -抗糜蛋白酶（ACT）、人溶菌酶、牛S - 100蛋白和人结蛋白。在所研究的组织中，AT未显示任何免疫反应性。除了1例MFH外，所有犬类MFH和其他具有“组织细胞样”特征的软组织肿瘤对溶菌酶染色阳性，而对S - 100染色阴性。7例犬类MFH和MFH样肿瘤中有6例结蛋白染色呈阳性，大多数非MFH肉瘤也是如此。大多数犬类和猫类MFH及MFH样肿瘤ACT染色呈阳性。犬类和猫类MFH及MFH样肿瘤中ACT染色的这些结果与人类MFH中的结果一致。犬类MFH和MFH样肿瘤的免疫组织化学结果与猫类不同。猫类MFH在溶菌酶和结蛋白染色方面与犬类MFH不同。
