一种两栖类水通道蛋白FA-CHIP的序列与功能表达：MIP家族的一个新成员
Sequence and functional expression of an amphibian water channel, FA-CHIP: a new member of the MIP family.
作者信息
Abrami L, Simon M, Rousselet G, Berthonaud V, Buhler J M, Ripoche P
机构信息
Département de Biologie Cellulaire et Moléculaire, CEA Saclay, Gif-sur-Yvette, France.
出版信息
Biochim Biophys Acta. 1994 Jun 1;1192(1):147-51. doi: 10.1016/0005-2736(94)90155-4.
A new member of the family of water channel proteins (aquaporin-CHIP) related to the major intrinsic protein (MIP) family is described. The cDNA coding for this amphibian CHIP was cloned from frog (Rana esculenta) urinary bladder, a model for the kidney collecting duct, using a RT-PCR cloning strategy. The encoded protein, designated FA-CHIP (frog aquaporin-CHIP), shows 77.4%, 42.4% and 35.6% identity with the three proteins now referred to as the aquaporins of the MIP family, i.e., human CHIP28, WCH-CD and gamma-TIP, respectively. Xenopus leavis injected with FA-CHIP cRNA exhibited a marked increase of the osmotic water permeability.
本文描述了一种与主要内在蛋白（MIP）家族相关的水通道蛋白家族（水通道蛋白-CHIP）的新成员。编码这种两栖动物CHIP的cDNA是从青蛙（食用蛙）膀胱（肾脏集合管的模型）中，采用逆转录-聚合酶链反应（RT-PCR）克隆策略克隆得到的。编码的蛋白，命名为FA-CHIP（青蛙水通道蛋白-CHIP），与目前称为MIP家族水通道蛋白的三种蛋白，即人类CHIP28、WCH-CD和γ-TIP，分别具有77.4%、42.4%和35.6%的同一性。注射了FA-CHIP cRNA的非洲爪蟾的渗透水通透性显著增加。
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