Di Prisco G V, Dubuc R, Grillner S
Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.
J Comp Neurol. 1994 Apr 1;342(1):23-34. doi: 10.1002/cne.903420104.
In order to determine if reticulospinal neurons involved in the control of locomotion and responsive to exogenously applied 5-hydroxytryptamine (5-HT) are innervated by fibers that contain serotonin, the serotoninergic innervation of reticulospinal neurons, identified by retrograde labeling with fluorescein-conjugated dextran-amine (FDA), was investigated by immunohistochemistry in the lamprey brainstem. A widespread distribution of 5-HT immunoreactive (5-HT-ir) fibers was seen within the basal plate of the brainstem, an area containing reticulospinal somata and dendritic aborizations. Numerous 5-HT varicose fibers were found in close relation to large reticulospinal cell bodies, particularly in the middle and anterior rhombencephalic reticular nuclei (MRRN and ARRN). Some of these reticulospinal somata were surrounded by a very dense pericellular 5-HT innervation. 5-HT-ir fibers were also seen in other brain structures that are known to influence reticulospinal neurons such as the rhombencephalic alar plate containing sensory relay interneurons, cranial nerves (III-X), cerebellum, and tectum. These findings suggest that, as in the spinal cord, motor behavior controlled by reticulospinal neurons may be subject to a serotoninergic modulation.
为了确定参与运动控制并对外源性应用的5-羟色胺（5-HT）有反应的网状脊髓神经元是否由含5-羟色胺的纤维支配，我们通过免疫组织化学方法在七鳃鳗脑干中研究了用荧光素偶联葡聚糖胺（FDA）逆行标记鉴定的网状脊髓神经元的5-羟色胺能神经支配。在脑干基板内可见广泛分布的5-羟色胺免疫反应性（5-HT-ir）纤维，该区域包含网状脊髓神经元的胞体和树突分支。在大型网状脊髓细胞体附近发现了许多5-HT曲张纤维，特别是在中脑和前脑桥网状核（MRRN和ARRN）中。其中一些网状脊髓神经元胞体被非常密集的细胞周围5-HT神经支配所包围。在其他已知影响网状脊髓神经元的脑结构中也可见5-HT-ir纤维，如含有感觉中继中间神经元的脑桥翼板、脑神经（III-X）、小脑和顶盖。这些发现表明，与脊髓一样，由网状脊髓神经元控制的运动行为可能受到5-羟色胺能调制。
