一氧化氮在乙酰胆碱诱导的人子宫动脉舒张中起主要作用。
Predominant role for nitric oxide in the relaxation induced by acetylcholine in human uterine artery.
作者信息
Jovanović A, Grbović L, Tulić I
机构信息
Department of Pharmacology, University Medical Centre, Medical Faculty, Belgrade, Yugoslavia.
出版信息
Hum Reprod. 1994 Mar;9(3):387-93. doi: 10.1093/oxfordjournals.humrep.a138514.
The effect of acetylcholine on the isolated human uterine artery rings was investigated. Acetylcholine (10(-10) M to 6 x 10(-5) M) induced concentration- and endothelium-dependent relaxation (pD2 = 7.4 +/- 0.02, maximal response was 77.5 +/- 6.3% of relaxation induced by papaverine at 3 x 10(-4) M) of the pre-contracted arterial segments. Indomethacin (10(-5) M), diethylcarbamazine (10(-4) M) and tetra-ethylammonium (3 x 10(-4) M) had no effects on acetylcholine-evoked relaxation. Methylene blue (10(-5) M) and NG-monomethyl-L-arginine (L-NMMA) (3 x 10(-6) to 3 x 10(-5) M) antagonized relaxation induced by acetylcholine. The inhibition of endothelium-dependent relaxation by L-NMMA (10(-5) M) was reversed by L-arginine (10(-5) M) but not by D-arginine (10(-4) M). It is concluded that in uterine artery acetylcholine induces endothelium-dependent relaxation of isolated uterine artery is probably mediated via endothelial nitric oxide formation.
研究了乙酰胆碱对离体人子宫动脉环的作用。乙酰胆碱（10⁻¹⁰ M至6×10⁻⁵ M）可引起预收缩动脉段浓度依赖性和内皮依赖性舒张（pD2 = 7.4±0.02，最大反应为3×10⁻⁴ M罂粟碱诱导舒张的77.5±6.3%）。吲哚美辛（10⁻⁵ M）、乙胺嗪（10⁻⁴ M）和四乙铵（3×10⁻⁴ M）对乙酰胆碱诱发的舒张无影响。亚甲蓝（10⁻⁵ M）和N⁃单甲基⁃L⁃精氨酸（L⁃NMMA）（3×10⁻⁶至3×10⁻⁵ M）拮抗乙酰胆碱诱导的舒张。L⁃精氨酸（10⁻⁵ M）可逆转L⁃NMMA（10⁻⁵ M）对内皮依赖性舒张的抑制作用，而D⁃精氨酸（10⁻⁴ M）则不能。结论是，子宫动脉中乙酰胆碱诱导的离体子宫动脉内皮依赖性舒张可能是通过内皮一氧化氮的形成介导的。
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