Lau J Y, Davis G L
Department of Medicine, University of Florida, Gainesville 32610.
J Med Virol. 1994 Mar;42(3):268-71. doi: 10.1002/jmv.1890420313.
A rapid technique for the detection of hepatitis C virus (HCV) RNA in liver section using nonisotopic in situ hybridization was described. Only 3 of the 10 patients seropositive for antibody to HCV and HCV RNA had detectable HCV RNA in the hepatocytes (1%, 2%, and 15% of cells positive). No specific signal was detected in sinusoidal, biliary epithelial, and mononuclear cells. The positive hepatocytes were scattered in the liver lobule with occasional clusters. The positive signal was confined to the cytoplasmic compartment. These data support the previous observation of the hepatocyte tropism of HCV.
描述了一种使用非同位素原位杂交技术在肝切片中快速检测丙型肝炎病毒（HCV）RNA的方法。在10例抗HCV抗体和HCV RNA血清学阳性的患者中，只有3例在肝细胞中检测到可检测的HCV RNA（阳性细胞分别为1%、2%和15%）。在窦状隙、胆管上皮细胞和单核细胞中未检测到特异性信号。阳性肝细胞散在于肝小叶中，偶尔成簇。阳性信号局限于细胞质区室。这些数据支持了先前关于HCV肝细胞嗜性的观察结果。
