Saito S, Kidd G J, Trapp B D, Dawson T M, Bredt D S, Wilson D A, Traystman R J, Snyder S H, Hanley D F
Department of Anesthesiology/Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland 21287-7839.
Neuroscience. 1994 Mar;59(2):447-56. doi: 10.1016/0306-4522(94)90608-4.
We describe the distribution and characteristics of nitric oxide synthase-containing neurons in rat spinal cord using a polyclonal affinity-purified antibody against rat cerebellar nitric oxide synthase. Numerous neurons were stained throughout the entire rostrocaudal extent of the spinal cord. Cell bodies, dendrites and axons stained in a uniform manner. Nitric oxide synthase immunoreactivity was intense in neurons of laminae I-IV and X throughout the entire spinal cord. Neurons in the intermediolateral cell column of the thoracic and lumbar spinal cord were also intensely stained for nitric oxide synthase. The sacral cord demonstrated substantial nitric oxide synthase immunostaining within lamina VII. For the entire cord, scattered neurons in laminae V, VI, VII, and VIII were weakly positive. In addition, punctate nitric oxide synthase staining throughout laminae I, III and surrounding some large motor neurons in the ventral horn suggested the presence of nitric oxide synthase at synapses. Axons and dendritic terminals located in the gray and white matter were also stained. The majority of nitric oxide synthase positive neurons in the intermediolateral cell column were double-labelled by subcutaneously injected FluoroGold confirming that these cells were preganglionic autonomic neurons. Most NADPH-diaphorase-stained neurons were also nitric oxide synthase-positive. The distribution of nitric oxide synthase-containing neurons in spinal cord suggests that nitric oxide plays a role in spinal cord neurotransmission including: preganglionic sympathetic and parasympathetic, somatosensory, visceral sensory and possibly motor pathways. In particular, the autonomic nervous system appears enriched with nitric oxide synthase immunoreactivity. The precise role of each neuron type remains to be demonstrated in physiologic and pathophysiologic paradigms.
我们使用针对大鼠小脑一氧化氮合酶的多克隆亲和纯化抗体，描述了大鼠脊髓中含一氧化氮合酶神经元的分布和特征。在脊髓的整个前后范围内都有大量神经元被染色。细胞体、树突和轴突均以均匀的方式被染色。在整个脊髓中，I - IV层和X层的神经元中一氧化氮合酶免疫反应强烈。胸段和腰段脊髓中间外侧细胞柱中的神经元也被强烈染色以显示一氧化氮合酶。骶段脊髓在VII层内显示出大量的一氧化氮合酶免疫染色。在整个脊髓中，V、VI、VII和VIII层中的散在神经元呈弱阳性。此外I、III层以及腹角一些大型运动神经元周围的点状一氧化氮合酶染色表明突触处存在一氧化氮合酶。位于灰质和白质中的轴突和树突终末也被染色。中间外侧细胞柱中大多数一氧化氮合酶阳性神经元通过皮下注射荧光金进行双重标记，证实这些细胞是节前自主神经元。大多数还原型辅酶II - 黄递酶染色的神经元也是一氧化氮合酶阳性。脊髓中含一氧化氮合酶神经元的分布表明一氧化氮在脊髓神经传递中发挥作用，包括：节前交感和副交感神经、躯体感觉、内脏感觉以及可能的运动通路。特别是，自主神经系统似乎富含一氧化氮合酶免疫反应性。每种神经元类型的确切作用仍有待在生理和病理生理模式中得到证实。
