通过分析羊水细胞聚合酶链反应扩增的基因组DNA对胎儿进行人类血小板抗原-1（Zw）分型。

Human platelet antigen-1 (Zw) typing of fetuses by analysis of polymerase chain reaction-amplified genomic DNA from amniocytes.

作者信息

Simsek S, Christiaens G C, Kanhai H H, Beekhuis J R, Bleeker P M, Vlekke A B, Goldschmeding R, von dem Borne A E

机构信息

Department of Immunological Haematology, University of Amsterdam, The Netherlands.

出版信息

Transfus Med. 1994 Mar;4(1):15-9. doi: 10.1111/j.1365-3148.1994.tb00238.x.

DOI:10.1111/j.1365-3148.1994.tb00238.x

PMID:7516786

Abstract

Prenatal typing for the human platelet antigens-1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA-1 incompatibility in which the father is heterozygous for the HPA-1a antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele-specific restriction enzyme analysis on polymerase chain reaction (PCR)-amplified DNA purified from amniocytes. This assays allows early second trimester typing for HPA-1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.

摘要

对人类血小板抗原-1（HPA）进行产前分型，可在父亲为HPA-1a抗原杂合子的HPA-1不相容情况下，识别有新生儿同种免疫性血小板减少症（NAITP）风险的胎儿。为此目的，采用诊断性脐带穿刺术和胎儿血小板表型分析。我们对从羊水中提取的经聚合酶链反应（PCR）扩增并纯化的DNA进行等位基因特异性限制性酶切分析。该检测方法可在孕中期早期对HPA-1等位基因进行分型。我们能够确定三个有风险胎儿的基因型。羊膜穿刺术导致的医源性胎儿丢失率低于脐带穿刺术。因此，这项技术是NAITP产前管理中一项受欢迎的补充方法。