Nakamura K, Nishiguchi M, Minemura M, Honda K, Yamane K
Institute of Biological Sciences, University of Tsukuba, Ibarak, Japan.
Microbiology (Reading). 1994 Mar;140 ( Pt 3):493-8. doi: 10.1099/00221287-140-3-493.
Using a DNA fragment of Bacillus subtilis scRNA as a probe, a Bacillus brevis gene encoding the small cytoplasmic RNA was cloned and characterized. B. brevis scRNA consists of 273 nucleotides; the sequence has comparatively low homology (approximately 70%) with other Bacillus sequences. Phylogenetic analysis indicated that B. brevis forms a line of descent distinct from other Bacillus species. However, despite the low overall homology, both functional nucleotide sequence and secondary structural features defined among signal recognition particle (SRP) RNA family members were well conserved.
以枯草芽孢杆菌小细胞质RNA（scRNA）的DNA片段为探针，克隆并鉴定了一个编码短小芽孢杆菌小细胞质RNA的基因。短小芽孢杆菌scRNA由273个核苷酸组成；其序列与其他芽孢杆菌序列的同源性相对较低（约70%）。系统发育分析表明，短小芽孢杆菌形成了一条与其他芽孢杆菌物种不同的谱系。然而，尽管总体同源性较低，但信号识别颗粒（SRP）RNA家族成员中定义的功能性核苷酸序列和二级结构特征都得到了很好的保守。
